Suzhou Chien-Shiung Institute of Technology, 1 Jianxiong Road, Suzhou 215411, China.
State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
Biosensors (Basel). 2022 Apr 20;12(5):260. doi: 10.3390/bios12050260.
An abnormal level of dopamine (DA) is usually related to neurological disorders, including Parkinson's disease. Herein, cubic-shaped, Prussian blue nanoparticle-supported MoS nanocomposites (MoS-CPBNPs) were prepared as peroxidase-like nanozymes for the label-free, colorimetric detection of DA. As expected, the as-prepared MoS-CPBNPs nanozymes have outstanding peroxidase-like mimicking activity, which can catalyze 3,3',5,5'-Tetramethylbenzidine (TMB) to generate blue, oxidized TMB in the presence of hydrogen peroxide (HO). DA can inhibit the oxidation of TMB, which causes blue solutions to fade and become colorless. According to this phenomenon, the developed colorimetric sensor can qualitatively and quantitatively analyze DA ranging from 0 to 300 μM with a detection limit of 0.09 μM. In addition, the high recovery and low relative standard deviation for practical DA determination suggested that this colorimetric sensor has potential for application in biological biosensing and diagnostic fields.
多巴胺（DA）水平异常通常与神经紊乱有关，包括帕金森病。在此，我们制备了立方体形普鲁士蓝纳米颗粒负载的 MoS 纳米复合材料（MoS-CPBNPs）作为过氧化物酶样纳米酶，用于无标记比色法检测 DA。正如预期的那样，所制备的 MoS-CPBNPs 纳米酶具有出色的过氧化物酶样模拟活性，可在过氧化氢（HO）存在下催化 3,3',5,5'-四甲基联苯胺（TMB）生成蓝色、氧化的 TMB。DA 可以抑制 TMB 的氧化，导致蓝色溶液褪色变为无色。根据这一现象，开发的比色传感器可以定性和定量分析 0 到 300 μM 范围内的 DA，检测限为 0.09 μM。此外，实际 DA 测定的高回收率和低相对标准偏差表明，这种比色传感器在生物生物传感和诊断领域具有应用潜力。
