Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, Hangzhou, 311121, P. R. China.
State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, P. R. China.
Chem Commun (Camb). 2019 Sep 24;55(77):11567-11570. doi: 10.1039/c9cc04416g.
A small series of fluorescent lysosome-targeting probes based on the BODIPY fluorophore and containing morpholine and nitro groups were rationally designed. These probes emitted light from green to NIR wavelengths, and provided specificity for imaging the lysosomes of hypoxic cells. The electron-withdrawing nitrophenyl group at the meso position was found to lead to highly efficient nonradiative decay of the S1 state, and hence a recovery of fluorescence when reduction of the nitro group occurred under hypoxic conditions.
一组基于 BODIPY 荧光团的、含有吗啉和硝基的荧光靶向溶酶体探针被合理设计出来。这些探针的发光波长从绿色到近红外，特异性地用于成像缺氧细胞的溶酶体。在中位引入吸电子的硝基苯基，导致 S1 态的非辐射衰减非常有效，因此在缺氧条件下硝基被还原时，荧光会恢复。
