Silswal Akshay, Kanojiya Ashutosh, Koner Apurba Lal
Bionanotechnology Lab, Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal, India.
Front Chem. 2022 Mar 11;10:840297. doi: 10.3389/fchem.2022.840297. eCollection 2022.
The cellular physiochemical properties such as polarity, viscosity, and pH play a critical role in cellular homeostasis. The dynamic change of lysosomal viscosity in live cells associated with different environmental stress remains enigmatic and needs to be explored. We have developed a new class of Julolidine-based molecular viscometers with an extended conjugation to probe the lysosomal viscosity in live cells. High biocompatibility, pH tolerance, and the fluorogenic response with far red-emission (>600 nm) properties make these molecular viscometers suitable for live-cell fluorescence imaging in . Among these probes, is specifically designed to target lysosomes simple modification. The real-time monitoring of lysosomal viscosity change under cellular stress was achieved. We believe that such a class of molecule viscometers has the potential to monitor lysosomal health in pathogenic conditions.
细胞的生理化学性质，如极性、粘度和pH值，在细胞内稳态中起着关键作用。与不同环境应激相关的活细胞中溶酶体粘度的动态变化仍然是个谜，需要进行探索。我们开发了一类新型的基于 julolidine 的分子粘度计，其具有扩展的共轭结构，用于探测活细胞中的溶酶体粘度。高生物相容性、pH耐受性以及远红光发射（>600 nm）的荧光响应特性使这些分子粘度计适用于活细胞荧光成像。在这些探针中， 通过简单修饰被专门设计用于靶向溶酶体。实现了细胞应激下溶酶体粘度变化的实时监测。我们相信，这类分子粘度计有潜力在致病条件下监测溶酶体的健康状况。
