Key Laboratory of Oral Clinical Medicine, the Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
Medical Research Center, the Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
Ann N Y Acad Sci. 2020 Feb;1462(1):65-78. doi: 10.1111/nyas.14190. Epub 2019 Sep 8.
Oral squamous cell carcinoma (OSCC) is one of the most common cancer types, with a high annual incidence. Although chemotherapy contributes to suppressing OSCC tumorigenesis, the available treatments result in poor prognosis because of local recurrence and regional lymph node metastasis. Thus, it is necessary to discover novel and safe drugs with greater effectiveness and fewer side effects. Fucoidan is a component of the cell wall of brown seaweed that has been shown to produce a wide range of biological activities. The present study aimed to investigate the effectiveness of fucoidan in treating OSCC. In in vitro studies, we found that fucoidan inhibited OSCC growth and suppressed migration and invasion of OSCC cells. In addition, the potential interaction between fucoidan and filamin A (FLNA)-derived circular RNA (circFLNA) was predicted using bioinformatics databases and then confirmed in OSCC samples and cell lines. Indeed, fucoidan increased the expression of circFLNA in OSCC cell lines. Furthermore, both fucoidan and circFLNA could mediate the expression of key proteins related to cell growth, apoptosis, migration, and invasion. In conclusion, our research demonstrated that fucoidan might be considered as a potential natural drug in the treatment of OSCC patients by targeting circFLNA.
口腔鳞状细胞癌 (OSCC) 是最常见的癌症类型之一，年发病率较高。尽管化疗有助于抑制 OSCC 肿瘤发生，但由于局部复发和区域淋巴结转移，现有的治疗方法导致预后不佳。因此，有必要发现具有更大疗效和更少副作用的新型安全药物。褐藻中的岩藻聚糖是一种细胞壁成分，已被证明具有广泛的生物活性。本研究旨在探讨岩藻聚糖在治疗 OSCC 中的有效性。在体外研究中，我们发现岩藻聚糖抑制 OSCC 生长并抑制 OSCC 细胞的迁移和侵袭。此外，使用生物信息学数据库预测了岩藻聚糖与细丝蛋白 A (FLNA)衍生的环状 RNA (circFLNA)之间的潜在相互作用，并在 OSCC 样本和细胞系中得到了证实。事实上，岩藻聚糖增加了 OSCC 细胞系中 circFLNA 的表达。此外，岩藻聚糖和 circFLNA 都可以调节与细胞生长、凋亡、迁移和侵袭相关的关键蛋白的表达。总之，我们的研究表明，岩藻聚糖通过靶向 circFLNA 可能被认为是治疗 OSCC 患者的一种潜在天然药物。
