没食子酸预处理对大鼠肾脏缺血再灌注损伤的保护作用
The Protective Role of Gallic Acid Pretreatment On Renal Ischemia-reperfusion Injury in Rats.
作者信息
Ahmadvand Hassan, Yalameha Banafsheh, Adibhesami Glavizh, Nasri Maryam, Naderi Negar, Babaeenezhad Esmaeel, Nouryazdan Negar
机构信息
Razi Herbal Medicine Research Center, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
出版信息
Rep Biochem Mol Biol. 2019 Apr;8(1):42-48.
BACKGROUND
Renal ischemia-reperfusion injury (RIR) occurs when there is a temporary restriction of blood flow to the kidneys followed by an influx of blood, re-oxygenating the tissues. This occurs as a severe complication of major surgery. This process causes significant damage to the tissues and is responsible for the development of acute kidney injury (AKI), a life-threatening condition with high mortality rates. Here, we evaluated the potential protective effects of the antioxidant, gallic acid (GA), on RIR in an rat model.
METHODS
Adult male Sprague Dawley rats were randomly divided into three groups: group 1 (control, n = 8), group 2 (Ischemia-reperfusion (IR) with no-treatment, n = 7), and group 3 (IR + daily GA 100 mg/kg i.p, n = 7). The abdomens of the rats in the control group were opened during the surgical procedure, then sutured closed. GA pretreatment began daily 15 days prior to inducing RIR. To induce RIR, the umbilical arteries were obstructed on both sides and clamped with mild pressure for 45 min. Following the 45 min ischemia, the clamps were removed to allow for the induction of reperfusion. The reperfusion phase was 24 hours.
RESULTS
Following IR, the serum levels of urea and creatinine significantly increased compared to the controls. Pretreatment with GA was observed to reduce urea and creatinine levels following IR. However, this decrease was not statistically significant. The serum and renal levels of malondialdehyde (MDA) in the IR group was significantly elevated compared to the control group. Conversely, glutathione (GSH) levels and the activity of glutathione peroxidase (GPX) significantly decreased in the IR group compared to controls. Our findings show GA pretreatment to significantly improve the levels of renal MDA, serum GSH, and GPX activity following RIR.
CONCLUSION
Our findings highlight the protective role for GA in mitigating the damage caused by RIR and its applications as a potential treatment.
背景
肾缺血再灌注损伤（RIR）发生于肾脏血流暂时受限后又有血流涌入，使组织重新获得氧合之时。这是大手术的一种严重并发症。此过程会对组织造成显著损伤，并导致急性肾损伤（AKI）的发生，AKI是一种死亡率很高的危及生命的病症。在此，我们在大鼠模型中评估了抗氧化剂没食子酸（GA）对RIR的潜在保护作用。
方法
成年雄性Sprague Dawley大鼠随机分为三组：第1组（对照组，n = 8）、第2组（缺血再灌注（IR）未治疗组，n = 7）和第3组（IR + 每日腹腔注射GA 100 mg/kg，n = 7）。对照组大鼠在手术过程中打开腹腔，然后缝合关闭。GA预处理在诱导RIR前15天开始每日进行。为诱导RIR，双侧脐动脉被阻断并以轻度压力夹闭45分钟。45分钟缺血后，移除夹子以诱导再灌注。再灌注阶段为24小时。
结果
IR后，与对照组相比，血清尿素和肌酐水平显著升高。观察到GA预处理可降低IR后的尿素和肌酐水平。然而，这种降低无统计学意义。与对照组相比，IR组血清和肾脏中的丙二醛（MDA）水平显著升高。相反，与对照组相比，IR组中的谷胱甘肽（GSH）水平和谷胱甘肽过氧化物酶（GPX）活性显著降低。我们的研究结果表明，GA预处理可显著改善RIR后肾脏MDA水平、血清GSH水平和GPX活性。
结论
我们的研究结果突出了GA在减轻RIR所致损伤方面的保护作用及其作为潜在治疗方法的应用。
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