Bergau Nick, Maul Stephan, Rujescu Dan, Simm Andreas, Navarrete Santos Alexander
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany.
Department of Psychiatry, Psychotherapy and Psychosomatics, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.
Front Neurosci. 2019 Aug 21;13:871. doi: 10.3389/fnins.2019.00871. eCollection 2019.
The profile of 122 metabolites in the cerebrospinal fluid (CSF) of patients suffering from Alzheimer's disease (AD) and controls was studied. Among the 122 metabolites analyzed, 61 could be detected. Statistically significant differences between the AD and control group were only detected for metabolites of the glycolysis. Thus, accurate quantification of 11 glycolytic metabolites was done. We detected a significant reduction of five of them, namely phosphoenolpyruvate, 2-phosphoglycerate, 3-phosphoglycerate, pyruvate and dihydroxyacetone phosphate in the AD CSF compared to controls. These results correlate with the known reduction of glucose metabolism in the brain of patients with AD and indicate that metabolic analysis of the central carbon metabolism can be a potential tool in AD diagnostic. Although the Receiver operating characteristic (ROC) analyses of the metabolites do not reach the level of the diagnostic informativity of AD biomarkers, the combination of specific glycolysis metabolites with the established biomarkers may lead to an improvement in sensitivity and specificity.
对阿尔茨海默病（AD）患者和对照组的脑脊液（CSF）中的122种代谢物进行了分析。在所分析的122种代谢物中，有61种可以检测到。仅在糖酵解代谢物中检测到AD组和对照组之间具有统计学意义的差异。因此，对11种糖酵解代谢物进行了准确的定量分析。我们检测到其中五种代谢物在AD患者脑脊液中的含量显著降低，分别是磷酸烯醇丙酮酸、2-磷酸甘油酸、3-磷酸甘油酸、丙酮酸和磷酸二羟丙酮。这些结果与AD患者大脑中已知的葡萄糖代谢降低相关，并表明中枢碳代谢的代谢分析可能是AD诊断中的一种潜在工具。尽管对这些代谢物的受试者工作特征（ROC）分析未达到AD生物标志物的诊断信息水平，但特定糖酵解代谢物与已确立的生物标志物的组合可能会提高敏感性和特异性。
