Zhang Ye, He Yun, Deng Ning, Chen Yan, Huang Jiecong, Xie Wei
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, 510515, China.
Department of Traditional Chinese Medicine, The Third People's Hospital of Yunnan Province, Kunming, Yunnan, 650011, China.
Transl Neurosci. 2019 Aug 24;10:235-240. doi: 10.1515/tnsci-2019-0038. eCollection 2019.
Resveratrol(RES) is a natural polyphenol which possesses an anti-depressant effect. However, the mechanisms of its anti-depressant effect remain unclear. The aim of the study is to investigate the potential mechanisms in the neuro-protective efficiency in the corticosterone-induced pheochromacytoma 12 (PC12) cells.
PC12 cells were treated with 200 μM of corticosterone in the absence or presence of different concentrations of RES for 24 h. Then, cell viability was measured by Cell Counting Kit-8 assay. Apoptosis of PC12 cells was measured by Annexin V-FITC and Propidium iodide (PI) labelling. The expression of apoptosis-related proteins including Bax, Bcl-2, caspase-3 was determined by western blotting.
The results showed that treatment with 200 μM of corticosterone induced cytotoxicity in PC12 cells. However, different concentrations of RES (2.5μmol/L, 5μmol/L and 10 μmol/L) significantly increased the cell viability, suppressed the apoptosis of PC12 cells, down-regulated Bax and caspase-3 protein expression, and up-regulated Bcl-2 protein expression, compared to the model group (<0.05).
Resveratrol has a protective effect on corticosterone-induced neurotoxicity in PC12 cells, which may be related to the apoptosis via inhibition of apoptosis-related proteins and displays the antidepressant-like effect.
白藜芦醇（RES）是一种具有抗抑郁作用的天然多酚。然而，其抗抑郁作用的机制尚不清楚。本研究旨在探讨其在皮质酮诱导的嗜铬细胞瘤12（PC12）细胞中神经保护作用的潜在机制。
将PC12细胞在不存在或存在不同浓度RES的情况下用200μM皮质酮处理24小时。然后，通过细胞计数试剂盒-8测定法测量细胞活力。通过膜联蛋白V-异硫氰酸荧光素（Annexin V-FITC）和碘化丙啶（PI）标记来测量PC12细胞的凋亡。通过蛋白质免疫印迹法测定包括Bax、Bcl-2、半胱天冬酶-3在内的凋亡相关蛋白的表达。
结果表明，用200μM皮质酮处理可诱导PC12细胞产生细胞毒性。然而，与模型组相比，不同浓度的RES（2.5μmol/L、5μmol/L和10μmol/L）显著提高了细胞活力，抑制了PC12细胞的凋亡，下调了Bax和半胱天冬酶-3蛋白的表达，并上调了Bcl-2蛋白的表达（<0.05）。
白藜芦醇对皮质酮诱导的PC12细胞神经毒性具有保护作用，这可能与通过抑制凋亡相关蛋白的凋亡有关，并显示出抗抑郁样作用。
