大豆中该基因家族的全基因组鉴定与表达分析

Genome-wide identification and expression analysis of the gene family in soybean ().

作者信息

Wang Yongbin, Jiang Zhenfeng, Li Zhenxiang, Zhao Yuanling, Tan Weiwei, Liu Zhaojun, Cui Shaobin, Yu Xiaoguang, Ma Jun, Wang Guangjin, Li Wenbin

机构信息

Key Laboratory of Soybean Biology in Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin, Heilongjiang, China.

Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, China.

出版信息

PeerJ. 2019 Aug 21;7:e7509. doi: 10.7717/peerj.7509. eCollection 2019.

BACKGROUND

VQ proteins, the plant-specific transcription factors, are involved in plant development and multiple stresses; however, only few articles systematic reported the genes in soybean.

METHODS

In total, we identified 75 genes, which were classified into 7 groups (I-VII). Conserved domain analysis indicated that gene family members all contain the domains. genes from the same evolutionary branches of soybean shared similar motifs and structures. Promoter analysis revealed that -elements related to stress responses, phytohormone responses and controlling physical as well as reproductive growth. Based on the RNA-seq and qRT-PCR analysis, genes were showed expressing in nine tissues, suggesting their putative function in many aspects of plant growth and development as well as response to stress in .

RESULTS

This study aims to understand the roles of genes in various development processes and their expression patterns in responses to stimuli. Our results provide basic information in identification and classification of genes. Further experimental analysis will allows us to know the functions of participation in plant growth and stress responses.

背景

VQ蛋白是植物特有的转录因子，参与植物发育和多种胁迫反应；然而，仅有少数文章系统报道了大豆中的这些基因。

方法

我们共鉴定出75个基因，这些基因被分为7组（I - VII）。保守结构域分析表明，该基因家族成员均含有这些结构域。来自大豆同一进化分支的基因具有相似的基序和结构。启动子分析显示，存在与胁迫反应、植物激素反应以及控制营养生长和生殖生长相关的元件。基于RNA测序和qRT-PCR分析，这些基因在9个组织中均有表达，表明它们在大豆植物生长发育的多个方面以及对胁迫的响应中可能具有功能。