Zhong Yan, Wang Ping, Zhang Xiaohui, Cheng Zong-Ming
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
School of Life Science, Nanjing University, Nanjing 210023, China.
Int J Genomics. 2021 Dec 17;2021:4066394. doi: 10.1155/2021/4066394. eCollection 2021.
Genes encoding VQ motif-containing (VQ) transcriptional regulators and WRKY transcription factors can participate separately or jointly in plant growth, development, and abiotic and biotic stress responses. In this study, 222 VQ and 645 WRKY genes were identified in six species. Based on phylogenetic tree topologies, the VQ and WRKY genes were classified into 13 and 32 clades, respectively. Therefore, at least 13 VQ gene copies and 32 WRKY gene copies were present in the genome of the common ancestor of the six species. Similar small Ks value peaks for the VQ and WRKY genes suggest that the two gene families underwent recent duplications in the six studied species. The majority of the Ka/Ks ratios were less than 1, implying that most of the VQ and WRKY genes had undergone purifying selection. Pi values were significantly higher in the VQ genes than in the WRKY genes, and the VQ genes therefore exhibited greater nucleotide diversity in the six species. Forty-one of the VQ genes were predicted to interact with 44 of the WRKY genes, and the expression levels of some predicted VQ-WRKY interacting pairs were significantly correlated. Differential expression patterns of the VQ and WRKY genes suggested that some might be involved in regulating aphid resistance in and fruit development in .
编码含VQ基序转录调节因子（VQ）和WRKY转录因子的基因可单独或共同参与植物生长、发育以及非生物和生物胁迫反应。在本研究中，在六个物种中鉴定出222个VQ基因和645个WRKY基因。基于系统发育树拓扑结构，VQ和WRKY基因分别被分为13个和32个进化枝。因此，六个物种的共同祖先基因组中至少存在13个VQ基因拷贝和32个WRKY基因拷贝。VQ和WRKY基因相似的小Ks值峰值表明这两个基因家族在六个被研究物种中经历了近期的复制。大多数Ka/Ks比值小于1，这意味着大多数VQ和WRKY基因经历了纯化选择。VQ基因的Pi值显著高于WRKY基因，因此VQ基因在六个物种中表现出更大的核苷酸多样性。预测41个VQ基因与44个WRKY基因相互作用，并且一些预测的VQ-WRKY相互作用对的表达水平显著相关。VQ和WRKY基因的差异表达模式表明，其中一些基因可能参与调控[物种名称1]的抗蚜虫性和[物种名称2]的果实发育。
