Liang Tianyu, Yang Guang, Ma Yunxia, Yao Qingzhi, Ma Yuan, Ma Hui, Hu Yang, Yang Ying, Wang Shaoxiong, Pan Yiyong, Li Gangtie
College of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
PeerJ. 2019 Aug 22;7:e7526. doi: 10.7717/peerj.7526. eCollection 2019.
The seasonal dynamics of microbial diversity within the rhizosphere of L. var. in the hinterland of the Otindag Sandy Land of China were investigated using high-throughput sequencing of bacterial 16S rRNA genes and fungal ITS region sequences. A significant level of bacterial and fungal diversity was observed overall, with detection of 7,676 bacterial Operational Taxonomic Units (OTUs) belonging to 40 bacteria phyla and 3,582 fungal OTUs belonging to six phyla. Proteobacteria, Actinobacteria, and Firmicutes were the dominant bacterial phyla among communities, while Ascomycota, Basidiomycota, and Zygomycota were the dominant phyla of fungal communities. Seasonal changes influenced the α-diversity and β-diversity of bacterial communities within elm rhizospheres more than for fungal communities. Inferred functional analysis of the bacterial communities identified evidence for 41 level two KEGG (Kyoto Encyclopedia of Genes and Genomes) orthology groups, while guild-based analysis of the fungal communities identified eight ecological guilds. Metabolism was the most prevalent bacterial functional group, while saprotrophs prevailed among the identified fungal ecological guilds. Soil moisture and soil nutrient content were important factors that affected the microbial community structures of elm rhizospheres across seasons. The present pilot study provides an important baseline investigation of elm rhizosphere microbial communities.
利用细菌16S rRNA基因和真菌ITS区域序列的高通量测序技术，对中国浑善达克沙地腹地黄柳根际微生物多样性的季节动态进行了研究。总体上观察到了显著水平的细菌和真菌多样性，共检测到属于40个细菌门的7676个细菌操作分类单元（OTU）和属于6个门的3582个真菌OTU。变形菌门、放线菌门和厚壁菌门是群落中主要的细菌门，而子囊菌门、担子菌门和接合菌门是真菌群落的主要门。季节变化对黄柳根际细菌群落α多样性和β多样性的影响大于真菌群落。对细菌群落的推断功能分析确定了41个二级KEGG（京都基因与基因组百科全书）直系同源组的证据，而基于功能类群的真菌群落分析确定了8个生态功能类群。代谢是最普遍的细菌功能组，而腐生菌在已确定的真菌生态功能类群中占主导地位。土壤湿度和土壤养分含量是影响黄柳根际微生物群落结构的重要因素。本初步研究为黄柳根际微生物群落提供了重要的基线调查。
