Wang Hai H, Chu Hong L, Dou Qing, Xie Qing Z, Tang Ming, Sung Chang K, Wang Chun Y
College of Forestry, Northwest A&F University, Yangling, China.
Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon, South Korea.
Front Microbiol. 2018 Aug 27;9:1930. doi: 10.3389/fmicb.2018.01930. eCollection 2018.
The temporal distribution patterns of bacterial communities, as an important group in mountain soil, are affected by various environmental factors. To improve knowledge regarding the successional seasonal dynamics of the mountain soil bacterial communities, the rhizospheric soil of a 30-year-old natural secondary forest, located in the high-altitude (1900 m a.s.l.) of the temperate Qinling Mountains, was sampled and studied during four different seasons. The bacterial community composition and structure in the rhizospheric soil were studied using an Illumina MiSeq Sequencing platform. Furthermore, the edaphic properties and soil enzymatic activities (urease, phosphatase, and catalase) were measured in order to identify the main impact factors on the soil bacterial community. According to the results, all of the edaphic properties and soil enzymatic activities were significantly affected by the seasonal changes, except for the C/N ratio. Although the biomasses of soil bacterial communities increased during the summer and autumn (warm seasons), their Shannon diversity and Pielou's evenness were decreased. Proteobacteria, Acidobacteria, Actinobacteria, Planctomycetes, and Bacteroidetes were the predominant bacterial groups in all of the soil samples, and the genera of , as well as an unclassified member of the Ktedonobacteria were the keystone taxa. The composition and structure of soil bacterial communities were strongly impacted by the edaphic properties, especially the temperature, moisture, ammoniacal nitrogen, available phosphorus and total phosphorus which were the crucial factors to drive the temporal distribution of the soil bacterial community and diversity. In conclusion, the soil temperature, moisture and the nutrients N and P were the crucial edaphic factors for shaping the rhizospheric soil bacterial communities as season and climate change in a . forest of Qinling Mountains.
细菌群落的时间分布模式作为山地土壤中的一个重要群体，受到多种环境因素的影响。为了增进对山地土壤细菌群落演替季节动态的了解，对位于温带秦岭高海拔地区（海拔1900米）的一片30年生天然次生林的根际土壤在四个不同季节进行了采样和研究。使用Illumina MiSeq测序平台研究了根际土壤中的细菌群落组成和结构。此外，还测量了土壤性质和土壤酶活性（脲酶、磷酸酶和过氧化氢酶），以确定对土壤细菌群落的主要影响因素。结果表明，除碳氮比外，所有土壤性质和土壤酶活性均受到季节变化的显著影响。虽然土壤细菌群落的生物量在夏季和秋季（温暖季节）有所增加，但其香农多样性和皮洛均匀度却降低了。变形菌门、酸杆菌门、放线菌门、浮霉菌门和拟杆菌门是所有土壤样品中的主要细菌类群， 属以及Ktedonobacteria的一个未分类成员是关键分类单元。土壤细菌群落的组成和结构受到土壤性质的强烈影响，特别是温度、湿度、氨氮、有效磷和总磷，这些是驱动土壤细菌群落时间分布和多样性的关键因素。总之，在秦岭山区的一片次生林中，土壤温度、湿度以及养分氮和磷是塑造根际土壤细菌群落随季节和气候变化的关键土壤因素。
