Nagvenkar Anjani P, Perelshtein Ilana, Piunno Ylenia, Mantecca Paride, Gedanken Aharon
Department of Chemistry and Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Department of Earth and Environmental Sciences, Research Center POLARIS, University of Milano-Bicocca, Milan 20126, Italy.
ACS Omega. 2019 Aug 12;4(9):13631-13639. doi: 10.1021/acsomega.9b00181. eCollection 2019 Aug 27.
Most antibacterial agents demand their action in the form of a liquid for compatibility and ease of use in biosystems, which are mainly composed of biological fluids. Controlling the colloidal stability of metal oxide nanocolloids, in parallel with minimizing the effect of using a large amount of surfactant on their biocidal activity and cytotoxicity, remains a challenge. Here, we address the stability of nanocolloids of ZnO and CuO in the presence of polymer surfactants and the influence of the surface capping on their antibacterial activity and cytotoxicity. The metal oxide nanoparticles (NPs) were synthesized sonochemically in a single step and tested against both Gram-negative and Gram-positive to validate their biocidal efficacy. Cytotoxicity studies were performed on human alveolar epithelial cells. Polyethylene glycol- and polyvinyl alcohol-capped NPs are observed to show the minimum cytotoxicity whereas polyethylene imine-capped and pristine metal oxide NPs are toxic to the mammalian cells. The cytotoxic and antibacterial properties of the stable nanocolloids displayed an inverse relation, highlighting the role and significance of the polymer capping. The nontoxic biocidal nanocolloids showed an effective antibacterial efficacy of 99.9% in 2 h.
大多数抗菌剂需要以液体形式发挥作用，以便在主要由生物流体组成的生物系统中具有兼容性和易于使用性。在控制金属氧化物纳米胶体的胶体稳定性的同时，尽量减少大量表面活性剂对其杀菌活性和细胞毒性的影响，仍然是一个挑战。在这里，我们研究了在聚合物表面活性剂存在下氧化锌和氧化铜纳米胶体的稳定性，以及表面封端对其抗菌活性和细胞毒性的影响。金属氧化物纳米颗粒（NPs）通过超声化学一步合成，并针对革兰氏阴性菌和革兰氏阳性菌进行测试，以验证其杀菌效果。对人肺泡上皮细胞进行了细胞毒性研究。观察到聚乙二醇和聚乙烯醇封端的纳米颗粒显示出最小的细胞毒性，而聚乙烯亚胺封端的和原始的金属氧化物纳米颗粒对哺乳动物细胞有毒。稳定纳米胶体的细胞毒性和抗菌性能呈现反比关系，突出了聚合物封端剂的作用和重要性。无毒杀菌纳米胶体在2小时内显示出99.9%的有效抗菌效果。
