Kinoshita S, Seki T, Liu T F, Kushida T
Department of Physics, Osaka University, Japan.
J Photochem Photobiol B. 1988 Sep;2(2):195-208. doi: 10.1016/1011-1344(88)80003-4.
The fluorescence properties of hematoporphyrin (Hp) and its derivative (HpD) were investigated in leukemia cells and in normal lymphocytes under a microscope, and the results were compared with those in solution. The spectra and the time behaviour of Hp (or HpD) fluorescence in living cells were found to be almost the same as those in Hp solution of very high concentration. This implies that Hp is much more concentrated in the cells than in the medium. It was also found that irradiation with intense light easily gives rise to a photoproduct which gives an additional peak in the fluorescence spectrum. Possible methods to increase the sensitivity of cancer detection and localization are discussed.
在显微镜下研究了血卟啉（Hp）及其衍生物（HpD）在白血病细胞和正常淋巴细胞中的荧光特性，并将结果与溶液中的结果进行了比较。发现活细胞中Hp（或HpD）荧光的光谱和时间行为与高浓度Hp溶液中的几乎相同。这意味着Hp在细胞中的浓度比在培养基中高得多。还发现强光照射容易产生一种光产物，该光产物在荧光光谱中产生一个额外的峰。讨论了提高癌症检测和定位灵敏度的可能方法。
