Yi Ruokun, Chen Xi, Li Wenfeng, Mu Jianfei, Tan Fang, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing China.
Chongqing Engineering Research Center of Functional Food Chongqing University of Education Chongqing China.
Food Sci Nutr. 2020 Aug 13;8(9):5160-5171. doi: 10.1002/fsn3.1821. eCollection 2020 Sep.
Insect tea is consumed as a health beverage in China. The insect tea primary leaf (ITPL) is rich in bioactive substances, which are also used as traditional Chinese medicine. This study investigated the role of ITPL in reducing the oxidative response induced by D-galactose in mice. Mice were intraperitoneally injected with D-galactose to induce oxidative damage. The effect of ITPL was tested by pathological observation, serum detection with kits, quantitative polymerase chain reaction, and Western blot. The experimental results show that ITPL increased the thymus, brain, heart, liver, spleen, and kidney indices of oxidized mice. ITPL increased superoxide dismutase, glutathione peroxidase, and glutathione levels and reduced nitric oxide and malondialdehyde levels in the serum, liver, and spleen in oxidative damaged mice. The pathological observations show that ITPL reduced the oxidative damage of the liver and spleen in mice induced with D-galactose. Simultaneously, ITPL upregulated mRNA expression of neuronal nitric oxide synthase, endothelial nitric oxide synthase, cuprozinc-superoxide dismutase, manganese superoxide dismutase, catalase, heme oxygenase-1, nuclear factor-erythroid 2 related factor 2, γ-glutamylcysteine synthetase, and NAD(P)H dehydrogenase [quinone] 1, and downregulated the expression of inducible nitric oxide synthase in the liver and spleen of oxidized mice. ITPL had beneficial preventive effects on the oxidative damage caused by D-galactose in mice and was more effective as an antioxidant than vitamin C. The component analysis test by high-performance liquid chromatography indicated that ITPL contained the following seven compounds: neochlorogenic acid, cryptochlorogenic acid, rutin, kaempferin, isochlorogenic acid B, isochlorogenic acid A, and hesperidin. ITPL is a plant with excellent antioxidant activities derived from its bioactive substances.
在中国，虫茶被当作一种健康饮品饮用。虫茶原叶富含生物活性物质，也被用作中药。本研究调查了虫茶原叶在减轻D-半乳糖诱导的小鼠氧化反应中的作用。给小鼠腹腔注射D-半乳糖以诱导氧化损伤。通过病理观察、试剂盒血清检测、定量聚合酶链反应和蛋白质免疫印迹法来测试虫茶原叶的效果。实验结果表明，虫茶原叶提高了氧化损伤小鼠的胸腺、脑、心脏、肝脏、脾脏和肾脏指数。虫茶原叶提高了氧化损伤小鼠血清、肝脏和脾脏中的超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽水平，并降低了一氧化氮和丙二醛水平。病理观察表明，虫茶原叶减轻了D-半乳糖诱导的小鼠肝脏和脾脏的氧化损伤。同时，虫茶原叶上调了神经元型一氧化氮合酶、内皮型一氧化氮合酶、铜锌超氧化物歧化酶、锰超氧化物歧化酶、过氧化氢酶、血红素加氧酶-１、核因子相关因子2、γ-谷氨酰半胱氨酸合成酶和NAD(P)H脱氢酶［醌］1的mRNA表达，并下调了氧化损伤小鼠肝脏和脾脏中诱导型一氧化氮合酶的表达。虫茶原叶对D-半乳糖引起的小鼠氧化损伤具有有益的预防作用，并且作为抗氧化剂比维生素C更有效。高效液相色谱法的成分分析测试表明，虫茶原叶含有以下七种化合物：新绿原酸、隐绿原酸、芦丁、山柰酚、异绿原酸B、异绿原酸A和橙皮苷。虫茶原叶是一种因其生物活性物质而具有优异抗氧化活性的植物。
