Kot Barbara, Kwiatek Kinga, Janiuk Justyna, Witeska Małgorzata, Pękala-Safińska Agnieszka
Department of Microbiology, Faculty of Natural Sciences, Siedlce University of Natural Sciences and Humanities, 14 Bolesława Prusa Str., 08-110 Siedlce, Poland.
Department of Animal Physiology, Faculty of Natural Sciences, Siedlce University of Natural Sciences and Humanities, 14 Bolesława Prusa Str., 08-110 Siedlce, Poland.
Pathogens. 2019 Sep 8;8(3):142. doi: 10.3390/pathogens8030142.
Antimicrobial activities of phytochemicals--cinnamaldehyde (TC), ferulic acid (FA), -coumaric acid (-CA), caffeic acid (CA), chlorogenic acid (CHA), essential oil (TO), essential oil (ECO), and oil (TTO) against species-were assessed. Growth of all subsp. and almost all strains was inhibited by TC at concentration 0.01 mg/mL, and for most strains minimal inhibitory concentrations (MIC) ranged from 0.01 to 0.19 mg/mL. The inhibitory effect of TC against subsp. was comparable to the effect of oxytetracycline, and in the case of subsp. and was higher compared to gentamicin. MIC of FA, -CA, and CA for most strains ranged from 1.56 to 3.12 mg/mL, and MIC values of TO for most strains ranged from 0.39 to 0.78 mg/mL. TO and TC at the concentrations below ½ MIC values used in mixtures exhibited strong synergism. ECO and TC showed synergy in mixture of ⅛ MIC of ECO and ¼ MIC of TC. TC and TO exhibited the strongest inhibitory and bactericidal effect against investigated species, and they are a promising alternative to the use of antibiotics in controlling the growth of these fish pathogens.
评估了植物化学物质——肉桂醛（TC）、阿魏酸（FA）、对香豆酸（-CA）、咖啡酸（CA）、绿原酸（CHA）、百里香精油（TO）、牛至精油（ECO）和丁香精油（TTO）对[具体物种]的抗菌活性。在浓度为0.01mg/mL时，TC抑制了所有[具体亚种]和几乎所有[具体菌株]的生长，并且对于大多数[具体菌株]，最小抑菌浓度（MIC）范围为0.01至0.19mg/mL。TC对[具体亚种]的抑制作用与土霉素相当，并且在[具体亚种]和[具体情况]中，与庆大霉素相比更高。大多数菌株对FA、-CA和CA的MIC范围为1.56至3.12mg/mL，大多数菌株对TO的MIC值范围为0.39至0.78mg/mL。在混合物中使用低于½ MIC值的浓度时，TO和TC表现出强烈的协同作用。在ECO的⅛ MIC和TC的¼ MIC的混合物中，ECO和TC显示出协同作用。TC和TO对所研究的[具体物种]表现出最强的抑制和杀菌作用，并且它们是控制这些鱼类病原体生长中使用抗生素的有前途的替代品。
