Pękala-Safińska Agnieszka, Tkachenko Halyna, Kurhaluk Natalia, Buyun Lyudmyla, Osadowski Zbigniew, Honcharenko Vitaliy, Prokopiv Andriy
Department of Fish Diseases, National Veterinary Research Institute, 24-100 Puławy, Poland.
Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, 76-200 Słupsk, Poland.
J Vet Res. 2021 Jan 26;65(1):59-66. doi: 10.2478/jvetres-2021-0007. eCollection 2021 Mar.
The most frequently isolated bacteria in Polish aquaculture are of the genus; also pathogenic to human fish consumers, they cause substantial economic losses, and require antibiotic therapy to treat. Antibiotic residues in animal-derived food provoke concern. The aim of the study was to appraise the antimicrobial activity of ethanolic extracts of plant species against strains.
Leaves of 41 species were collected from two Ukrainian botanic gardens. They were crushed, washed, homogenized in ethanol and centrifuged, and the supernatants were applied in the Kirby-Bauer disc-diffusion method to assess the susceptibility to them of , , and subsp. isolates confirmed as K886, K825, and St30 strains. Analogous assessment was also made of these bacteria's susceptibility to sulfonamides, quinolones, tetracyclines, and one amphenicol. Data were analysed statistically.
The majority of the extracts considerably inhibited bacterial growth, being susceptible to 14 species, subsp. to 13, and to 10.
Treatment with plant extracts has promise as an alternative to antibiotic therapy. Botanic gardens may offer new sources of plant-derived agents with a broad spectrum of biological and antimicrobial action. Further research will be useful to broaden knowledge of therapeutic potential.
波兰水产养殖中最常分离出的细菌属于该属;它们对食用鱼类的人类也具有致病性,会造成重大经济损失,且需要抗生素治疗。动物源性食品中的抗生素残留引发了人们的关注。本研究的目的是评估植物物种乙醇提取物对菌株的抗菌活性。
从乌克兰的两个植物园收集了41种植物的叶子。将叶子粉碎、洗涤,在乙醇中匀浆并离心,然后将上清液应用于 Kirby-Bauer 纸片扩散法,以评估它们对确诊为K886、K825和St30菌株的、、和亚种分离株的敏感性。还对这些细菌对磺胺类、喹诺酮类、四环素类和一种氯霉素的敏感性进行了类似评估。对数据进行了统计分析。
大多数提取物显著抑制细菌生长,对14种植物敏感,亚种对13种敏感,对10种敏感。
用植物提取物治疗有望成为抗生素治疗的替代方法。植物园可能会提供具有广泛生物和抗菌作用的植物源药物的新来源。进一步的研究将有助于拓宽对治疗潜力的认识。