Voronova А D, Stepanova O V, Valikhov M P, Chadin A V, Semkina А S, Abakumov M A, Reshetov I V, Chekhonin V P
Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2019 Aug;167(4):536-540. doi: 10.1007/s10517-019-04568-z. Epub 2019 Sep 9.
Olfactory ensheathing cells showed significant effects on the regeneration of the spinal cord in experimental models and in clinical trials. However, the use of these cells in the therapy of posttraumatic cysts of the spinal cord has not been studied. Cultures of human and rat olfactory mucosa were obtained according to the protocols developed by us. Passage 3-4 cultures are most enriched with olfactory ensheathing cells and are preferable for transplantation. We performed transplantation of 750,000 olfactory ensheathing cells into the region of modeled cysts. The therapeutic effect of human cells was more pronounced. The positive dynamics of recovery of motor activity in the hind limbs of rats can reflect regenerative processes in the spinal cord after transplantation of olfactory ensheathing cells into the region of posttraumatic cysts.
在实验模型和临床试验中，嗅鞘细胞对脊髓再生显示出显著效果。然而，这些细胞在创伤后脊髓囊肿治疗中的应用尚未得到研究。根据我们制定的方案获取了人和大鼠的嗅黏膜培养物。第3 - 4代培养物中嗅鞘细胞最为富集，适合用于移植。我们将750,000个嗅鞘细胞移植到模拟囊肿区域。人源细胞的治疗效果更为显著。大鼠后肢运动活动恢复的积极动态可以反映将嗅鞘细胞移植到创伤后囊肿区域后脊髓中的再生过程。
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