Voronova А D, Stepanova O V, Valikhov M P, Chadin A V, Dvornikov А S, Reshetov I V, Chekhonin V P
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia.
Bull Exp Biol Med. 2018 Mar;164(4):523-527. doi: 10.1007/s10517-018-4025-x. Epub 2018 Mar 5.
We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.
我们开发了一种用于从人嗅黏膜制备和培养嗅鞘细胞的优化方案。使用该方案，我们获得了富含人嗅鞘细胞的培养物。通过同时表达GFAP和p75NTR标志物进行的免疫荧光分析表明，在第3代和第4代培养物中，鞘细胞的含量最高（分别为94%和89.5%）。所开发的方案可推荐用于获取人鞘细胞的自体制剂，用于脊髓损伤的细胞治疗。
