School of Biological Science and Technology, University of Jinan, Jinan 250022, PR China.
College of Marine Science, Shandong University, Weihai 264209, PR China.
Int J Syst Evol Microbiol. 2019 Dec;69(12):3830-3836. doi: 10.1099/ijsem.0.003691.
A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped and aerobic bacterial strain (designated lm1) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong province, PR China). Strain lm1 grew between 15 and 45 °C (optimum, 40 °C). The pH range for growth was at pH 4.0-10.0 (optimum growth at pH 8.0). The range of NaCl concentration for the growth of strain lm1 was 0-4.0 % (w/v), with optimum growth at 1.0-2.0 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain lm1 belonged to the genus and was closely related to JCM 16873 (96.7 % 16S rRNA gene sequence similarity) and CCTCC AB2013083 (96.2 %). The average nucleotide identity value and the digital DNA-DNA hybridization value between strain lm1 and JCM 16873 were 73.4 and 14.3 %, respectively. The major cellular fatty acids of strain lm1 were Ccyclo ω8, Cω7 and C. Strain lm1 contained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and five unknown lipids. The G+C content of the genomic DNA was 64.4 mol%. Based on polyphasic taxonomic data, strain lm1 could be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is lm1 (=CCTCC AB2019071=KCTC 72156).
一株从丙烯氧化物皂化废水活性污泥中分离得到的革兰氏阴性、非孢子形成、非运动、短杆状、需氧细菌菌株(命名为 lm1)。菌株 lm1 在 15 到 45°C 之间生长(最适温度为 40°C)。其生长的 pH 值范围为 4.0-10.0(最适生长 pH 值为 8.0)。菌株 lm1 生长的 NaCl 浓度范围为 0-4.0%(w/v),最适生长浓度为 1.0-2.0%(w/v)。基于 16S rRNA 基因序列的系统发育分析表明,菌株 lm1 属于 属,与 JCM 16873(96.7% 16S rRNA 基因序列相似性)和 CCTCC AB2013083(96.2% 16S rRNA 基因序列相似性)最为密切相关。菌株 lm1 与 JCM 16873 的平均核苷酸同一性值和数字 DNA-DNA 杂交值分别为 73.4%和 14.3%。菌株 lm1 的主要细胞脂肪酸为 Ccyclo ω8、Cω7 和 C。菌株 lm1 含有 Q-10 作为主要的呼吸醌。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、双磷脂酰甘油、三种未鉴定的胺脂、一种未鉴定的糖脂和五种未知脂组成。基因组 DNA 的 G+C 含量为 64.4mol%。基于多相分类学数据,菌株 lm1 可归类为 属的一个新种的代表,建议将其命名为 sp. nov。模式菌株为 lm1(=CCTCC AB2019071=KCTC 72156)。