Biological and Environmental Sciences and Engineering Division (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004866.
A strictly aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium, designated strain R1DC9, was isolated from sediments of a mangrove stand on the Red Sea coast of Saudi Arabia via diffusion chamber cultivation. Strain R1DC9 grew at 20-40 °C (optimum, 37 °C), pH 6-10 (optimum, pH 8) and 3-11 % NaCl (optimum, 7-9 %) in the cultivation medium. The genome of R1DC9 was 4 661 901 bp long and featured a G+C content of 63.1 mol%. Phylogenetic analyses based on the 16S rRNA gene sequence and whole-genome multilocus sequence analysis using 120 concatenated single-copy genes revealed that R1DC9 represents a distinct lineage in the order and the phylum separated from the and families. R1DC9 displayed 90 and 89 % 16S rRNA gene sequence identities with DSM 4125 and KMM 6017, respectively. The predominant quinone was MK7. The polar lipids were phosphatidylethanolamine, two unknown phospholipids and two unknown lipids. The predominant cellular fatty acids were the saturated branch chain fatty acids iso-C, iso-C 3-OH and iso-C, along with a low percentage of the monounsaturated fatty acid C 5. Based on differences in phenotypic, physiological and biochemical characteristics from known relatives, and the results of phylogenetic analyses, R1DC9 (=KCTC 72349=JCM 33609=NCCB 100698) is proposed to represent a novel species in a new genus, and the name gen. nov., sp. nov. is proposed. The distinct phylogenetic lineage among the families in the order indicates that R1DC9 represents a new family for which the name fam. nov. is proposed.
一株严格需氧、革兰氏阴性、不运动、杆状的细菌，通过扩散室培养，从沙特阿拉伯红海沿岸的红树林沉积物中分离得到，命名为 R1DC9 菌株。R1DC9 菌株在培养介质中生长的温度为 20-40°C（最适温度为 37°C）、pH 值为 6-10（最适 pH 值为 8）和 3-11%NaCl（最适 7-9%NaCl）。R1DC9 的基因组长 4661901bp，G+C 含量为 63.1mol%。基于 16S rRNA 基因序列和使用 120 个串联的单拷贝基因的全基因组多位点序列分析的系统发育分析表明，R1DC9 代表了一个独特的分支，位于 和 目，与 和 科分离。R1DC9 与 DSM 4125 和 KMM 6017 的 16S rRNA 基因序列相似性分别为 90%和 89%。主要醌为 MK7。极性脂为磷脂酰乙醇胺、两种未知磷脂和两种未知脂。主要的细胞脂肪酸为饱和支链脂肪酸 iso-C、iso-C 3-OH 和 iso-C，以及少量的单不饱和脂肪酸 C 5。基于与已知亲缘关系的表型、生理和生化特征的差异以及系统发育分析的结果，R1DC9（=KCTC 72349=JCM 33609=NCCB 100698）被提议代表一个新属中的一个新种，建议采用新属名 gen. nov.，种名 sp. nov.。目内科之间的独特系统发育分支表明，R1DC9 代表了一个新科，建议采用新科名 fam. nov.。
