Research Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, 1160 Vienna, Austria.
Institute of Diagnostic Virology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, D-17493 Greifswald-Insel Riems, Germany.
Viruses. 2024 Jul 31;16(8):1232. doi: 10.3390/v16081232.
Here, we report the results of a monitoring study of bat viruses in Austria to strengthen the knowledge of circulating viruses in Austrian bat populations. In this study, we analyzed 618 oropharyngeal and rectal swab samples from 309 bats and 155 pooled tissue samples from dead bats. Samples were collected from 18 different bat species from multiple locations in Austria, from November 2015 to April 2018, and examined for astroviruses, bornaviruses, coronaviruses, hantaviruses, morbilliviruses, orthomyxoviruses (influenza A/C/D viruses), pestiviruses and rhabdoviruses (lyssaviruses) using molecular techniques and sequencing. Using RT-qPCR, 36 samples revealed positive or suspicious results for astroviruses, Brno-hantaviruses, and coronaviruses in nine different bat species. Further sequencing revealed correspondent sequences in five samples. In contrast, none of the tested samples was positive for influenza viruses A/C/D, bornaviruses, morbilliviruses, lyssaviruses, or pestiviruses.
在这里，我们报告了一项在奥地利监测蝙蝠病毒的研究结果，旨在加强对奥地利蝙蝠种群中循环病毒的了解。在这项研究中，我们分析了 618 份来自 309 只蝙蝠的口咽和直肠拭子样本，以及 155 份来自死亡蝙蝠的混合组织样本。样本采集自奥地利 18 个不同的蝙蝠物种，来自 2015 年 11 月至 2018 年 4 月的多个地点，使用分子技术和测序方法检测了星状病毒、博尔纳病毒、冠状病毒、汉坦病毒、麻疹病毒、正粘病毒（流感 A/C/D 病毒）、瘟病毒和弹状病毒（狂犬病病毒）。使用 RT-qPCR，在 9 种不同的蝙蝠物种中，36 个样本对星状病毒、布尔诺汉坦病毒和冠状病毒呈阳性或可疑结果。进一步的测序在 5 个样本中揭示了相应的序列。相比之下，在测试的样本中，没有一个样本对流感病毒 A/C/D、博尔纳病毒、麻疹病毒、狂犬病病毒或瘟病毒呈阳性。
