Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China.
FEMS Microbiol Lett. 2019 Aug 1;366(15). doi: 10.1093/femsle/fnz188.
Heat shock protein 70 (HSP70) is an evolutionarily conserved chaperone protein. However, the role of HSP70 in mycoparasitism is unclear. Clonostachys rosea shows great potential against plant fungal pathogens. An HSP70 encoding gene, crhsp, from C. rosea 67-1 was significantly upregulated during C. rosea parasitization of the sclerotia of Sclerotinia sclerotiorum. In the present study, we investigated the role of crhsp in mycoparasitism using gene knockout experiments. The results showed that disruption of crhsp had remarkabe effects on the morphological characteristics of C. rosea. In addition, the ability of C. rosea to parasitize sclerotia and control soybean Sclerotinia stem rot in the greenhouse was significantly reduced in the Δcrhsp mutant. The results indicated that crhsp is involved in C. rosea mycoparasitism and provide the basis for further study of the molecular mechanism of C. rosea mycoparasitism. This is the first report to demonstrate the involvement of the HSP70 gene in C. rosea mycoparasitism.
热休克蛋白 70（HSP70）是一种进化上保守的伴侣蛋白。然而，HSP70 在菌寄生中的作用尚不清楚。层出镰刀菌对植物真菌病原体表现出巨大的潜力。层出镰刀菌 67-1 中一个 HSP70 编码基因 crhsp 在层出镰刀菌寄生核盘菌菌核的过程中显著上调。在本研究中，我们通过基因敲除实验研究了 crhsp 在菌寄生中的作用。结果表明，crhsp 的破坏对层出镰刀菌的形态特征有显著影响。此外，Δcrhsp 突变体中，层出镰刀菌寄生菌核的能力和在温室中控制大豆菌核茎腐病的能力显著降低。这些结果表明 crhsp 参与了层出镰刀菌的菌寄生，并为进一步研究层出镰刀菌菌寄生的分子机制提供了依据。这是首次报道 HSP70 基因参与层出镰刀菌菌寄生。
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