Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.
Mol Plant Microbe Interact. 2020 Jul;33(7):902-910. doi: 10.1094/MPMI-03-20-0062-R. Epub 2020 Jun 2.
is a mycoparasite used for biocontrol of numerous fungal plant pathogens. Sequencing of the transcriptome of following mycoparasitization of the sclerotia of revealed significant upregulation of a mitogen-activated protein kinase (MAPK)-encoding gene, . Although MAPKs are known to regulate fungal growth and development, the function of in mycoparasitism is unclear. In this study, we investigated the role of in mycoparasitism through gene knockout and complementation. Deletion of had no influence on the morphological characteristics but could significantly reduce the mycoparasitic ability to sclerotia and biocontrol capacity to soybean Sclerotinia stem rot; complementation restored these abilities. Transcriptome analysis between Δ and the wild-type strain revealed numerous genes were significantly down-regulated after deletion, including cytochrome P450, transporters, and cell wall-degrading enzymes (CWDEs). Our findings indicate that influences mycoparasitism by regulation of genes controlling the activity of CWDEs or antibiotic production. This study provides a basis for further studies of the molecular mechanism of mycoparasitism.
是一种真菌寄生菌，用于生物防治许多真菌植物病原体。对 后菌核的转录组进行测序，发现一种丝裂原活化蛋白激酶（MAPK）编码基因 的表达显著上调。尽管 MAPK 已知可调节真菌的生长和发育，但 在 真菌寄生中的功能尚不清楚。在这项研究中，我们通过基因敲除和互补研究了 在 真菌寄生中的作用。 的缺失对 的形态特征没有影响，但可以显著降低对菌核的真菌寄生能力和对大豆茎溃疡病的生物防治能力； 的互补恢复了这些能力。Δ 和野生型菌株之间的转录组分析表明， 缺失后许多基因的表达显著下调，包括细胞色素 P450、转运蛋白和细胞壁降解酶（CWDE）。我们的研究结果表明， 通过调节控制 CWDE 活性或抗生素产生的基因来影响真菌寄生。这项研究为进一步研究 真菌寄生的分子机制提供了基础。
