Yu Shu-Fan, Sun Zhan-Bin, Li Shi-Dong, Hu Ya-Feng, Ren Qing, Xu Jia-Liang, Song Han-Jian, Sun Man-Hong
School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Fungi (Basel). 2023 Aug 18;9(8):861. doi: 10.3390/jof9080861.
is an excellent biocontrol fungus against numerous fungal plant pathogens. The cAMP signaling pathway is a crucial signal transduction pathway in fungi. To date, the role of the cAMP signaling pathway in mycoparasitism remains unknown. An adenylate cyclase-encoding gene, (an important component of the cAMP signaling pathway), was previously screened from 67-1, and its expression level was dramatically upregulated during the mycoparasitization of the sclerotia of . In this study, the function of in mycoparasitism was explored through gene knockout and complementation. The obtained results show that the deletion of influenced the growth rate and colony morphology of , as well as the tolerance to NaCl and HO stress. The mycoparasitic effects on the sclerotia of and the biocontrol capacity on soybean Sclerotinia stem rot in ∆ and ∆ were both attenuated compared with that of the wild-type strain and complementation transformants. To understand the regulatory mechanism of during mycoparasitism, transcriptomic analysis was conducted between the wild-type strain and knockout mutant. A number of biocontrol-related genes, including genes encoding cell wall-degrading enzymes and transporters, were significantly differentially expressed during mycoparasitism, suggesting that may be involved in mycoparasitism by regulating the expression of these DEGs. These findings provide insight for further exploring the molecular mechanism of mycoparasitism.
是一种针对多种真菌植物病原体的优秀生防真菌。cAMP信号通路是真菌中至关重要的信号转导通路。迄今为止，cAMP信号通路在菌寄生中的作用仍不清楚。先前从67-1中筛选出一个编码腺苷酸环化酶的基因（cAMP信号通路的一个重要组成部分），其表达水平在菌核菌菌核的菌寄生过程中显著上调。在本研究中，通过基因敲除和互补对其在菌寄生中的功能进行了探索。获得的结果表明，该基因的缺失影响了菌核菌的生长速率和菌落形态，以及对NaCl和H₂O₂胁迫的耐受性。与野生型菌株和互补转化体相比，缺失该基因的菌株对菌核菌菌核的菌寄生作用以及对大豆菌核病的生防能力均减弱。为了解该基因在菌寄生过程中的调控机制，对野生型菌株和敲除突变体进行了转录组分析。在菌寄生过程中，许多与生物防治相关的基因，包括编码细胞壁降解酶和转运蛋白的基因，存在显著差异表达，这表明该基因可能通过调控这些差异表达基因的表达参与菌寄生过程。这些发现为进一步探索菌寄生的分子机制提供了见解。
