Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang, 222005, China.
Microbiol Res. 2018 May;210:6-11. doi: 10.1016/j.micres.2018.03.002. Epub 2018 Mar 8.
Clonostachys chloroleuca 67-1 (formerly C. rosea 67-1) is a potential biocontrol fungus active against various fungal plant pathogens. From transcriptome sequencing of 67-1 parasitizing sclerotia of Sclerotinia sclerotiorum, we identified the transcription factor-encoding gene crtf that is significantly up-regulated during mycoparasitism. Transcription factors are widely distributed in fungi and involved in multiple biological processes. However, their role and regulatory mechanisms in mycoparasitism remain poorly understood. In this study, the function of crtf during 67-1 mycoparasitism was verified through gene knockout and complementation. The results showed that deletion of crtf did not influence fungal morphological characteristics, but the ability of the Δcrtf mutant to parasitize sclerotia and suppress soybean Sclerotinia white mold in the greenhouse was markedly diminished compared with the wild type strain. The biocontrol activity of Δcrtf recovered wild type levels when complemented with a plasmid expressing the crtf gene. These findings suggest that crtf plays a crucial role in C. chloroleuca mycoparasitism and provide insight into the molecular mechanisms underlying C. chloroleuca mycoparasitism on plant pathogenic fungi.
绿僵菌 67-1（原名玫烟色棒束孢 67-1）是一种对多种真菌植物病原体具有活性的潜在生防真菌。通过对 67-1 寄生菌核的转录组测序，我们鉴定了一个在菌寄生过程中显著上调表达的转录因子编码基因 crtf。转录因子在真菌中广泛分布，参与多种生物学过程。然而，它们在菌寄生中的作用和调控机制仍知之甚少。在这项研究中，通过基因敲除和互补实验验证了 crtf 在 67-1 菌寄生过程中的功能。结果表明，crtf 的缺失并不影响真菌的形态特征，但与野生型菌株相比，Δcrtf 突变体寄生菌核和抑制温室大豆菌核病的能力明显降低。当用表达 crtf 基因的质粒互补时，Δcrtf 的生防活性恢复到野生型水平。这些发现表明 crtf 在绿僵菌菌寄生中发挥着关键作用，并为绿僵菌对植物病原菌的菌寄生分子机制提供了新的见解。
