Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Buenos Aires, Argentina.
J Appl Microbiol. 2011 May;110(5):1177-86. doi: 10.1111/j.1365-2672.2011.04970.x. Epub 2011 Mar 8.
To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent.
The antagonistic activity of C. rosea BAFC3874 was determined in vitro by dual cultures. The strain effectively antagonized S. sclerotiorum in pot-grown lettuce and soybean plants. Antifungal activity assays of C. rosea BAFC3874 grown in culture established that the strain produced antifungal compounds against S. sclerotiorum associated with secondary metabolism. High mycelial growth inhibition coincided with sclerotia production inhibition. The C. rosea strain produced a microheterogeneous mixture of peptides belonging to the peptaibiotic family. Moreover, mycoparasitism activity was observed in the dual culture.
Clonostachys rosea strain BAFC3874 was proved to be an effective antagonist against the aggressive soil-borne pathogen S. sclerotiorum in greenhouse experiments. The main mechanisms involve peptaibiotic metabolite production and mycoparasitism activity.
Clonostachys rosea BAFC3874 may be a good fungal biological control agent against S. sclerotiorum. In addition, we were also able to isolate and identify peptaibols, an unusual family of compounds in this genus of fungi.
从抑病土壤中分离出的拮抗真菌菌株 Clonostachys rosea BAFC3874 对核盘菌的作用模式，并确定其作为生防剂的潜力。
通过双培养法测定 C. rosea BAFC3874 的拮抗活性。该菌株在盆栽生菜和大豆植株中有效拮抗核盘菌。C. rosea BAFC3874 培养物的抑菌活性测定表明，该菌株产生了与次级代谢相关的抗核盘菌化合物。高菌丝生长抑制与菌核产生抑制一致。该菌株产生了一种属于肽抗生素家族的微异质混合肽。此外，在双培养中观察到了真菌寄生作用。
温室试验证明，Clonostachys rosea 菌株 BAFC3874 是一种有效的核盘菌拮抗生物防治剂。主要机制包括肽抗生素代谢产物的产生和真菌寄生活性。
Clonostachys rosea BAFC3874 可能是一种对抗核盘菌的良好真菌生物防治剂。此外，我们还能够分离和鉴定肽抗生素，这是该真菌属中一类不常见的化合物。
