人类B淋巴细胞亚群上的IgA受体
Receptors for IgA on a subpopulation of human B lymphocytes.
作者信息
Gupta S, Platsoucas C D, Good R A
出版信息
Proc Natl Acad Sci U S A. 1979 Aug;76(8):4025-8. doi: 10.1073/pnas.76.8.4025.
Human B lymphocytes from peripheral blood and tonsils and leukemic B cells from patients with chronic lymphocytic leukemia (CLL) were examined for the presence of receptors for IgA. Receptors for IgA were present on 5-20% of B cells from peripheral blood of normal persons, 6-21% of B cells from tonsils removed because of chronic enlargement, and 4-10% of B cells from patients with CLL. Prior incubation of B lymphocytes in medium containing fetal calf serum was normally essential for expression of receptors for IgA. IgM and IgG receptors were also examined on B cells from tonsils and CLL. Receptors for IgM were present on a large proportion of B cells from tonsils and B cells from patients with CLL. Their detection also required incubation of B cells in medium containing fetal calf serum for 2-3 days. In one patient with CLL, IgM receptors were present on only a small proportion of B cells. No significant change in IgG receptors on B cells from tonsils or CLL was observed when freshly isolated B cells and cultured receptors on B cells progressively decreased as a consequence of shedding of free Fc receptors into the supernates.
检测了来自外周血和扁桃体的人B淋巴细胞以及慢性淋巴细胞白血病（CLL）患者的白血病B细胞中是否存在IgA受体。正常外周血中5%-20%的B细胞、因慢性肿大而切除的扁桃体中6%-21%的B细胞以及CLL患者中4%-10%的B细胞存在IgA受体。通常，B淋巴细胞预先在含有胎牛血清的培养基中孵育对于IgA受体的表达至关重要。还检测了扁桃体和CLL患者B细胞上的IgM和IgG受体。扁桃体B细胞和CLL患者B细胞中的大部分B细胞存在IgM受体。其检测也需要将B细胞在含有胎牛血清的培养基中孵育2-3天。在一名CLL患者中，只有一小部分B细胞存在IgM受体。当新鲜分离的B细胞和培养的B细胞上的受体由于游离Fc受体脱落到上清液中而逐渐减少时，未观察到扁桃体或CLL患者B细胞上的IgG受体有显著变化。
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