Parrott D M, Good R A, O'Neill G J, Gupta S
Proc Natl Acad Sci U S A. 1978 May;75(5):2392-5. doi: 10.1073/pnas.75.5.2392.
Locomotor activity of T cells with receptors for IgM and IgG, T cells without receptors for IgM or IgG, and T and non-T cells from human peripheral blood and human tonsils towards the chemoattractant casein was examined in modified Boyden chambers. T cells with receptors for IgG both from human tonsils and peripheral blood did not move in response to casein. T cells with receptors for IgM and those without receptors for IgM or IgG, on the other hand, moved very well toward casein and the distances were comparable to those achieved by T cells before separation. This difference in the locomotor activity of T cell subsets might explain their differential distribution in various lymphoid compartments. Separated T cells, cultured in medium supplemented with fetal calf serum, moved into the filters in response to casein. Prior culture of T cells in medium alone or in medium supplemented with human AB serum resulted in a reduction in the distance traveled in response to casein; however, the effect of AB serum was variable. Non-T cells from peripheral blood and B cells from tonsils responded poorly to casein.
在改良的博伊登小室中检测了来自人外周血和人扁桃体的具有IgM和IgG受体的T细胞、不具有IgM或IgG受体的T细胞以及T细胞和非T细胞对趋化因子酪蛋白的趋化活性。来自人扁桃体和外周血的具有IgG受体的T细胞对酪蛋白无反应。另一方面，具有IgM受体的T细胞以及不具有IgM或IgG受体的T细胞对酪蛋白趋化良好，趋化距离与分离前T细胞的趋化距离相当。T细胞亚群趋化活性的这种差异可能解释了它们在不同淋巴区室中的差异分布。在补充有胎牛血清的培养基中培养的分离T细胞对酪蛋白有反应，会迁移到滤膜上。单独在培养基中或在补充有人AB血清的培养基中预先培养T细胞，会导致其对酪蛋白的迁移距离缩短；然而，AB血清的效果是可变的。来自外周血的非T细胞和来自扁桃体的B细胞对酪蛋白反应较差。
