Reinherz E L, Kung P C, Goldstein G, Schlossman S F
Proc Natl Acad Sci U S A. 1979 Aug;76(8):4061-5. doi: 10.1073/pnas.76.8.4061.
A monoclonal antibody was produced to human peripheral blood T cells. This hybridoma antibody, termed OKT4, was reactive by indirect immunofluorescence with only 55-60% of the peripheral blood T cell population (OKT4+) and unreactive with normal B cells, null cells, and macrophages. The OKT4- T cell population contained the previously described TH2+ subset that has been shown to contain cytotoxic/suppressor cells. With cell-sorter separation of OKT4+ and OKT4- cells, it was shown that these T cell subsets were functionally discrete. Both gave proliferative responses with concanavalin A, alloantigens, and phytohemagglutinin although OKT4+ cells were much more responsive to the latter. OKT4+ cells alone responded to soluble antigens whereas OKT4- cells alone were cytotoxic after alloantigenic sensitization of unfractionated T cells. However, both OKT4+ and OKT4- cells were required during sensitization for optimal development of cytotoxicity. These data suggest that the OKT4+ subset represents a helper population and that the OKT4- subset contains the cytotoxic effector population. OKT4 could be a valuable reagent for determining alterations of these functional subsets in human diseases.
制备了一种针对人外周血T细胞的单克隆抗体。这种杂交瘤抗体称为OKT4，通过间接免疫荧光法，它仅与55%-60%的外周血T细胞群体（OKT4+）发生反应，而与正常B细胞、裸细胞和巨噬细胞不发生反应。OKT4-T细胞群体包含先前描述的TH2+亚群，该亚群已被证明含有细胞毒性/抑制性细胞。通过细胞分选仪分离OKT4+和OKT4-细胞，结果表明这些T细胞亚群在功能上是离散的。两者对刀豆球蛋白A、同种异体抗原和植物血凝素均有增殖反应，不过OKT4+细胞对后者的反应性更强。单独的OKT4+细胞对可溶性抗原有反应，而单独的OKT4-细胞在未分级T细胞经同种异体抗原致敏后具有细胞毒性。然而，在致敏过程中，为了使细胞毒性得到最佳发展，OKT4+和OKT4-细胞都需要。这些数据表明，OKT4+亚群代表辅助细胞群体，而OKT4-亚群包含细胞毒性效应细胞群体。OKT4可能是一种用于确定人类疾病中这些功能亚群变化的有价值试剂。
