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脊髓损伤期间谷氨酸释放的体外电化学测量。

Ex vivo electrochemical measurement of glutamate release during spinal cord injury.

作者信息

Nolan James K, Nguyen Tran N H, Fattah Mara, Page Jessica C, Shi Riyi, Lee Hyowon

机构信息

Weldon School of Biomedical Engineering, Birck Nanotechnology Center, Center for Implantable Devices, Purdue University, West Lafayette, IN, USA.

Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.

出版信息

MethodsX. 2019 Aug 23;6:1894-1900. doi: 10.1016/j.mex.2019.08.008. eCollection 2019.

DOI:10.1016/j.mex.2019.08.008
PMID:31508326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6727010/
Abstract

Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors.

摘要

创伤性脊髓损伤（SCI）后过量的谷氨酸释放与脊髓损伤程度的加重有关。然而，细胞外谷氨酸持续高水平背后的机制尚不清楚。安装在蔗糖双间隙记录室中的脊髓节段是创伤性脊髓损伤的既定模型。我们开发了一种方法，使用微型印刷谷氨酸生物传感器记录大鼠脊髓损伤后脊髓节段的谷氨酸释放。该方案对类似的谷氨酸生物传感器同样适用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/bce84bc08638/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/a50044ec1816/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/f396986214d8/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/9c11478ce46c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/df3148b5028f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/bce84bc08638/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/a50044ec1816/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/f396986214d8/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/9c11478ce46c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/df3148b5028f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0841/6727010/bce84bc08638/gr4.jpg

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本文引用的文献

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Real-time characterization of uptake kinetics of glioblastoma vs. astrocytes in 2D cell culture using microelectrode array.利用微电极阵列实时分析 2D 细胞培养中神经胶质瘤与星形胶质细胞摄取动力学的特征。
Analyst. 2018 Oct 8;143(20):4954-4966. doi: 10.1039/c8an01198b.
3
Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays.
利用碳纤维纳米复合材料传感器和微生物传感器阵列在体同时测量大鼠脑中的抗坏血酸和谷氨酸。
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J Neurotrauma. 2018 May 1;35(9):1057-1068. doi: 10.1089/neu.2017.5297. Epub 2018 Mar 13.
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Glutamate receptors, neurotoxicity and neurodegeneration.谷氨酸受体、神经毒性和神经退行性变。
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