脊髓损伤期间谷氨酸释放的体外电化学测量。

Ex vivo electrochemical measurement of glutamate release during spinal cord injury.

作者信息

Nolan James K, Nguyen Tran N H, Fattah Mara, Page Jessica C, Shi Riyi, Lee Hyowon

机构信息

Weldon School of Biomedical Engineering, Birck Nanotechnology Center, Center for Implantable Devices, Purdue University, West Lafayette, IN, USA.

Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.

出版信息

MethodsX. 2019 Aug 23;6:1894-1900. doi: 10.1016/j.mex.2019.08.008. eCollection 2019.

Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors.