Alipoor Shamila D, Adcock Ian M, Folkerts Gert, Garssen Johan, Mortaz Esmaeil
Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Priority Research Centre for Asthma and Respiratory Disease, Hunter Medical Research Institute, University of Newcastle, Newcastle, NSW, Australia; Airways Section, National Heart and Lung Institute, Imperial College London, London, United Kingdom.
Int J Mycobacteriol. 2019 Jul-Sep;8(3):218-222. doi: 10.4103/ijmy.ijmy_88_19.
Tuberculosis (TB) still remains a major health threat worldwide. The current TB diagnostics are suboptimal, and there is a high clinical need for identifying novel biomarkers of disease prevalence. Circulating exosomes have been currently attractive as novel biomarkers in a wide range of pathological conditions.
In this study, we performed bioinformatics analysis on the downstream targets of a dysregulated microRNA (miRNA) cluster induced by Bacillus Calmette-Guerin infection of human macrophages to provide greater understanding of their potential roles in disease pathogenesis.
Our analysis demonstrated that these dysregulated miRNAs have central roles in the host metabolic and energy pathways.
This suggests that the host miRNA network is perturbed by Mycobacterium to re-patterning host metabolism machinery to favor its intracellular survival. The dysregulated miRNAs can be delivered to local and distal cells by exosomes and thereby modulate their function.
结核病在全球范围内仍然是一个重大的健康威胁。当前的结核病诊断方法并不理想，临床上迫切需要识别疾病流行的新型生物标志物。循环外泌体目前作为多种病理状况下的新型生物标志物备受关注。
在本研究中，我们对人巨噬细胞经卡介苗感染诱导的失调微小RNA（miRNA）簇的下游靶点进行了生物信息学分析，以更深入了解它们在疾病发病机制中的潜在作用。
我们的分析表明，这些失调的miRNA在宿主代谢和能量途径中发挥核心作用。
这表明宿主miRNA网络受到分枝杆菌的干扰，从而重新塑造宿主代谢机制以利于其在细胞内生存。失调的miRNA可通过外泌体传递至局部和远端细胞，进而调节其功能。
