Denny T N, Kinsley K M, Benson J W, Bull R W
Vox Sang. 1979;37(4):244-50. doi: 10.1111/j.1423-0410.1979.tb02298.x.
This study reports the adaptation of an electronic particle-counting method for the enumeration of T cell and B cell rosettes of human lymphocytes. This method and the conventional hemocytometer method were compared in parallel on seventeen T cell and seven B cell rosette enumerations in a blind study design. All data were analyzed for statistical significance; correlation coefficents were calculated. There was significant agreement between both methods for T cells at p less than 0.001 (mean particle counter 80.6% +/-11.3 mean hemocytometer 76.7+/-8.2%) and for the B cells at p less than 0.002 (mean particle counter 22.5+/-9.7, mean hemocytometer 23.4+/-5.12). These results demonstrate that the electronic particle counter is useful as a rapid, convenient, semi-automated method for the enumeration of T cell and B cell rosettes.
本研究报告了一种电子粒子计数方法的适应性，用于对人淋巴细胞的T细胞和B细胞玫瑰花结进行计数。在一项盲法研究设计中，对17次T细胞玫瑰花结计数和7次B细胞玫瑰花结计数同时采用了该方法和传统的血细胞计数器方法进行比较。对所有数据进行统计学显著性分析；计算相关系数。两种方法在T细胞计数上具有显著一致性（p<0.001，粒子计数器平均值80.6%±11.3，血细胞计数器平均值76.7±8.2%），在B细胞计数上也具有显著一致性（p<0.002，粒子计数器平均值22.5±9.7，血细胞计数器平均值23.4±5.12）。这些结果表明，电子粒子计数器作为一种快速、便捷的半自动方法，可用于T细胞和B细胞玫瑰花结的计数。
