Kolberg J, Michaelsen T E
Acta Pathol Microbiol Scand C. 1979 Aug;87C(4):275-9.
Investigation was made of the mitogenic activation by Lathyrus odoratus (LATH) lectin and phytohaemagglutinin (PHA) of human T-enriched and non-T lympohcytes isolated by nylon wool filtration and sheep erythrocyte rosette depletion, respectively. LATH stimulated only weakly the T-enriched and non-T fractions as compared to PHA which strongly stimulated the T-enriched population and weakly stimulated the non-T cells. Removal of phagocytic cells from the unfractionated lymphocytes caused only a slight reduction in the response to LATH. The responses of mixtures of T-enriched and non-T lymphocytes were similar to that of unfractionated lymphocytes. The LATH lectin seems, therefore, to be neither a pure T nor a pure non-T cell mitogen, since both populations must be present for optimal response. When alpha-methyl-D-mannoside was added to unfractionated lymphocytes together with LATH, there was a strong inhibition of thymidine incorporation. D-glucose and N-acetyl-D-glucosamine diminished the stimulation effect to a lesser degree.
分别通过尼龙毛过滤和绵羊红细胞花环去除法分离出富含人T淋巴细胞和非T淋巴细胞，研究了香豌豆（LATH）凝集素和植物血凝素（PHA）对它们的促有丝分裂激活作用。与PHA相比，LATH对富含T细胞和非T细胞组分的刺激作用较弱，PHA强烈刺激富含T细胞群体，对非T细胞的刺激作用较弱。从未经分离的淋巴细胞中去除吞噬细胞只会使对LATH的反应略有降低。富含T细胞和非T淋巴细胞混合物的反应与未经分离的淋巴细胞相似。因此，LATH凝集素似乎既不是纯T细胞有丝分裂原，也不是纯非T细胞有丝分裂原，因为两个群体都必须存在才能产生最佳反应。当将α-甲基-D-甘露糖苷与LATH一起添加到未经分离的淋巴细胞中时，胸腺嘧啶核苷掺入受到强烈抑制。D-葡萄糖和N-乙酰-D-葡萄糖胺对刺激作用的减弱程度较小。
