Laboratory of Taishan Scholar Immunobiology, Shandong Agricultural University, Shandong, Tai'an 271018, PR China.
Vet Microbiol. 2010 Feb 24;141(1-2):68-72. doi: 10.1016/j.vetmic.2009.07.034. Epub 2009 Aug 8.
The clinical symptoms of infectious coryza are multiple and include nasal discharge, facial swelling, lacrimation, and anorexia. In general, the disease is not fatal to chicken; so, in experiments where animals are infected with Avibacterium paragallinarum, there have been debates about conducting the challenge model and evaluating the clinical signs. In this experiment, 150 chickens, aged 30 days, were randomly divided into different groups. Some groups were infected with the 'in-contact' challenge model and others with the artificial intrasinus-injection-route model. The bacterial isolates used were three field isolates of different serogroups of A. paragallinarum, including Hpg-8 (Page serovar A), CCM6075 (Page serovar B) and Hpg-668 (Page serovar C). During this study, a scoring system was used to record the clinical signs of the infected birds and evaluate the pathogenic diversity of the two models. The final results indicated that the 'in-contact' challenge model of the three isolates showed a more reliable representation of the natural infection under field conditions than the artificial intrasinus-injection-route model. Thus, on carrying out animal experiments, the effect of 'in-contact' challenge model is more accurate than the artificial intrasinus-injection-route model.
传染性鼻炎的临床症状多种多样，包括鼻分泌物、面部肿胀、流泪和食欲不振。一般来说，这种疾病对鸡并不致命；因此，在感染禽传染性鼻炎支气管败血波氏杆菌的动物实验中，关于是否进行挑战模型和评估临床症状一直存在争议。在本实验中，将 150 只 30 日龄的鸡随机分为不同的组。一些组感染了“接触”挑战模型，另一些组感染了人工鼻腔内注射模型。使用的细菌分离株是三种不同血清型的禽传染性鼻炎支气管败血波氏杆菌的田间分离株，包括 Hpg-8（Page 血清型 A）、CCM6075（Page 血清型 B）和 Hpg-668（Page 血清型 C）。在本研究中，使用评分系统记录感染鸟类的临床症状，并评估两种模型的致病多样性。最终结果表明，三种分离株的“接触”挑战模型比人工鼻腔内注射模型更能可靠地代表田间的自然感染。因此，在进行动物实验时，“接触”挑战模型的效果比人工鼻腔内注射模型更准确。
