Almashhadany Dhary Alewy, Mohammed Sarhang Hayyas, Mala Shaymaa Farooq, Odhah Mohammed Naji Ahmed, Ahmood Asaad Abdel Jalil
Department of Medical Laboratory Science, College of Science, Knowledge University, Erbil, Iraq.
Department of Pharmacy, College of Pharmacy, Knowledge University, Erbil, Iraq.
Ital J Food Saf. 2024 Jun 4;13(3):12521. doi: 10.4081/ijfs.2024.12521. eCollection 2024 Aug 5.
Bacterial pathogenic strains are as adaptable as strains and cause diverse intestinal and extraintestinal diseases in humans and other mammals worldwide. Red meat and its products are important hosts for many zoonotic diseases. This work was designed to investigate the frequency, serotypes, and antimicrobial resistance profile of isolated spp. in red meat (cattle, sheep, and goats) sold in Dhamar Governorate, Yemen. A total of 250 red meat samples were collected from the retail seller market between July and December 2022. All samples were transported immediately to the laboratory, subcultured on selective enrichment agar, and identified by serotyping and antimicrobial susceptibility tests disk diffusion methods. The results indicated 26 positive samples of out of the 250 samples (10.4%). Notably, isolates belong to ten various serotypes: Typhimurium 19.2%, Anatum 15.4%, Newport 11.5%, Enteritidis 11.5%, Muenchen 11.5%, Infantis 7.7%, Montevideo 7.7%, . Dublin 7.7%, Senftenberg 3.9%, and Arizona 3.9%. The antibiotic resistance profile revealed that 57.5%, 53.9%, and 53.9% of isolates are resistant to erythromycin, tetracycline, and norfloxacin, respectively. This resistance among spp. suggests a significant threat to health, which will in turn require an active safety measure and response. On the other hand, the seasonal variations "August and July" were found to be associated with an increased frequency of isolation.
细菌致病菌株与其他菌株一样具有适应性，可在全球范围内的人类和其他哺乳动物中引发各种肠道和肠道外疾病。红肉及其制品是许多人畜共患病的重要宿主。本研究旨在调查也门达马尔省销售的红肉（牛、羊和山羊）中分离出的沙门氏菌属的频率、血清型和抗菌药物耐药性概况。2022年7月至12月期间，从零售市场共采集了250份红肉样本。所有样本均立即送往实验室，在选择性富集琼脂上进行传代培养，并通过血清分型和抗菌药物敏感性试验（纸片扩散法）进行鉴定。结果显示，250份样本中有26份沙门氏菌阳性样本（10.4%）。值得注意的是，分离株属于十种不同的血清型：鼠伤寒沙门氏菌19.2%、鸭沙门氏菌15.4%、纽波特沙门氏菌11.5%、肠炎沙门氏菌11.5%、慕尼黑沙门氏菌11.5%、婴儿沙门氏菌7.7%、蒙得维的亚沙门氏菌7.7%、都柏林沙门氏菌7.7%、森夫滕贝格沙门氏菌3.9%和亚利桑那沙门氏菌3.9%。抗生素耐药性概况显示，分别有57.5%、53.9%和53.9%的分离株对红霉素、四环素和诺氟沙星耐药。沙门氏菌属中的这种耐药性对健康构成了重大威胁，这反过来需要积极的安全措施和应对措施。另一方面，发现“八月和七月”的季节变化与沙门氏菌分离频率增加有关。
