State Key laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Arch Virol. 2019 Dec;164(12):3111-3113. doi: 10.1007/s00705-019-04402-x. Epub 2019 Sep 17.
The complete genome of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli, was sequenced, revealing a linear double-stranded DNA genome of 48,939 bp with 46% GC content and protruding 150-bp 5' cohesive termini. The genome contained 85 open reading frames, 28 of which were annotated with known functions. No tRNA-encoding genes were detected. Phylogenetic analysis suggested that phage PHB17 is a novel phage of family Siphoviridae.
新型噬菌体 vB_EcoS_PHB17 的全基因组测序完成，该噬菌体可感染产志贺毒素大肠杆菌。基因组为线性双链 DNA，全长 48939bp，GC 含量为 46%，两端各有 150bp 的 5'粘性末端。基因组共包含 85 个开放阅读框，其中 28 个具有已知功能。未检测到 tRNA 编码基因。系统进化分析表明 PHB17 噬菌体属于肌尾噬菌体科。
