Division of Molecular Bacteriology, Institute of Medical Biotechnology and Experimental Oncology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland.
Department of Medical Microbiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medyków 18, 40-752, Katowice, Poland.
Arch Virol. 2021 Aug;166(8):2305-2310. doi: 10.1007/s00705-021-05092-0. Epub 2021 May 20.
A temperate siphovirus, phiCDKH01, was obtained from a clinical isolate of Clostridioides difficile. The phage genome is a 45,089-bp linear double-stranded DNA molecule with an average G+C content of 28.7%. It shows low similarity to known phage genomes, except for phiCD24-1. Genomic and phylogenetic analysis revealed that phiCDKH01 is a newly discovered phage. Sixty-six putative ORFs were predicted in the genome, 37 of which code for proteins with predicted functions. The phiCDKH01 prophage was localized in the host genome. The results of this study increase our knowledge about the genetic diversity of tailed phages.
从艰难梭菌的临床分离株中获得了一种温和的长尾噬菌体 phiCDKH01。该噬菌体基因组是一个 45089 碱基对的线性双链 DNA 分子，平均 G+C 含量为 28.7%。它与已知的噬菌体基因组相似度较低，除了 phiCD24-1 之外。基因组和系统发育分析表明，phiCDKH01 是一种新发现的噬菌体。基因组中预测了 66 个推定的 ORF，其中 37 个编码具有预测功能的蛋白质。phiCDKH01 前噬菌体定位于宿主基因组中。本研究的结果增加了我们对长尾噬菌体遗传多样性的了解。
