Peberdy J F
Department of Botany, University of Nottingham, UK.
Microbiol Sci. 1987 Apr;4(4):108-14.
Protoplasts have proved to be valuable tools in fungal genetics. Removal of the cell wall is clearly very significant because this organelle has an important role in cell-cell interactions. Using the molecular 'glue' polyethylene glycol or electrofusion methods, protoplasts can be fused in intraspecies and interspecies and intergeneric combinations. The technique has therefore opened up an important new area of study which has both fundamental and applied importance.
原生质体已被证明是真菌遗传学中有价值的工具。去除细胞壁显然非常重要，因为这个细胞器在细胞间相互作用中起着重要作用。利用分子“胶水”聚乙二醇或电融合方法，原生质体可以在种内、种间和属间组合中融合。因此，这项技术开辟了一个重要的新研究领域，具有基础和应用两方面的重要性。
