Arnau J, Oliver R P
School of Biological Sciences, University of East Anglia, Norwich, UK.
Curr Genet. 1993 May-Jun;23(5-6):508-11. doi: 10.1007/BF00312643.
Protoplasts of a pAN7-1-transformed isolate of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences and the expression of the hph gene was studied in 85 progeny. A very high proportion (80%) of the progeny contained vector sequences and 70% of the progeny were resistant to hygromycin. Inactivation of the hph gene occurred in eight progeny. Rearrangement of vector sequences can account for the inactivation: there is no evidence for any RIP-like mechanism. A number of novel bands were observed.
将携带载体串联重复序列的番茄叶霉病菌4号生理小种的pAN7 - 1转化分离株的原生质体与未转化的5号生理小种分离株进行融合，未对载体序列的存在进行选择。融合产物自发单倍体化。在85个后代中研究了pAN7 - 1序列的遗传和hph基因的表达。很高比例（80%）的后代含有载体序列，70%的后代对潮霉素具有抗性。8个后代中发生了hph基因的失活。载体序列的重排可以解释失活现象：没有证据表明存在任何类RIP机制。观察到一些新的条带。
