Department of Molecular Medicine, Immunology and General Pathology Unit, via Ferrata 9, University of Pavia, 27100 Pavia, Italy.
Department of Drug Sciences, viale Taramelli 12, University of Pavia, 27100 Pavia, Italy.
Molecules. 2019 Sep 17;24(18):3376. doi: 10.3390/molecules24183376.
is a medicinal plant used as anti-rheumatic and anti-inflammatory herbal remedy in Iraqi-Kurdistan. The aim of this study was to evaluate the anti-inflammatory activity of its extract and the isolated homoisoflavonoid () by studying the Ca-dependent NF-kB pathway. Nuclear translocation of p65 NF-kB subunit, as parameter of NF-kB activation, was visualized in human leukemic monocytes by immunofluorescence and Western blot analyses, after cell treatment with root extract or followed by Lipopolysaccharide stimulation. In parallel, Ca signals responsible for NF-kB activation and levels of inflammatory cytokines were investigated. LPS-induced p65 translocation was evident in monocytes and both treatments, in particular that with , were able to counteract this activation. Intracellular Ca oscillations were halted and the cytokine release reduced. These results confirm the traditional anti-inflammatory efficacy of and identify one of the molecules in the extract which appears to be responsible of this action.
是一种药用植物，在伊拉克库尔德地区被用作抗风湿和抗炎的草药疗法。本研究的目的是通过研究 Ca 依赖性 NF-kB 通路，评估其提取物和分离的异黄酮（）的抗炎活性。通过免疫荧光和 Western blot 分析，在人白血病单核细胞中观察到 NF-kB 激活的参数 p65 NF-kB 亚基的核易位，在细胞用根提取物或处理后，用脂多糖刺激。同时，研究了负责 NF-kB 激活的 Ca 信号和炎症细胞因子的水平。LPS 诱导的 p65 易位在单核细胞中明显，两种处理，特别是用处理，能够对抗这种激活。细胞内 Ca 振荡停止，细胞因子释放减少。这些结果证实了的传统抗炎功效，并确定了提取物中的一种分子，该分子似乎对此作用负责。
