通过核糖体 RNA 的下一代测序对温泉土壤的细菌群落结构进行分析。
Bacterial community structure analysis of a hot spring soil by next generation sequencing of ribosomal RNA.
机构信息
Department of Zoology & Biotechnology, HNB Garhwal University, Srinagar(Garhwal), Uttarakhand, India.
Department of Zoology & Biotechnology, HNB Garhwal University, Srinagar(Garhwal), Uttarakhand, India.
出版信息
Genomics. 2019 Sep;111(5):1053-1058. doi: 10.1016/j.ygeno.2018.06.008. Epub 2018 Jul 2.
In the present study the whole bacterial community structure of Tapovan hot spring soil located in the state of Uttarakhand, India was analysed through next generation sequencing. The hot spring soil is slightly alkaline in nature with abundance of sulphur. The spring soil was rich in various metallic and non-metallic elements required for bacterial survival. The community was found to comprise of 14 bacterial phyla with representation of members belonging to Firmicutes, Proteobacteria, Thermi, Bacteroidetes, Aquificae, Actinobacteria, chloroflexi, TM7, Fusobacteria etc. At the genus level Bacillus, Pseudomonas, Symbiobacterium, Thermus, Geobacillus, Anoxybacillus were found in abundance as compared to other genera like Flavobacterium, Ureibacillus, Clostridium, Meiothermus, Acinetobacter, Desulfotomaculum and Rheinheimera.
在本研究中，通过下一代测序分析了位于印度北阿坎德邦的塔普万温泉土壤的整个细菌群落结构。温泉土壤呈弱碱性，富含硫。该温泉土壤富含各种金属和非金属元素，是细菌生存所必需的。该群落由 14 个细菌门组成，其中包括厚壁菌门、变形菌门、热菌门、拟杆菌门、泉古菌门、放线菌门、绿弯菌门、TM7 门、梭杆菌门等成员的代表。在属水平上，与黄杆菌属、尿杆菌属、梭菌属、栖热菌属、地芽孢杆菌属、厌氧芽孢杆菌属和莱茵海默氏菌属等其他属相比，芽孢杆菌属、假单胞菌属、共生菌属、Thermus 属、Geobacillus 属和不动杆菌属更为丰富。
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