Engelbrecht Gerhard, Claassens Sarina, Mienie Charlotte M S, Fourie Hendrika
Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2520, South Africa.
Microorganisms. 2021 Aug 26;9(9):1813. doi: 10.3390/microorganisms9091813.
Soybean is among South Africa's top crops in terms of production figures. Over the past few years there has been increasingly more damage caused to local soybean by plant-parasitic nematode infections. The presence of (root-knot nematodes) and spp. (root lesion nematodes) in soybean fields can cripple the country's production, however, little is known about the soil microbial communities associated with soybean in relation to different levels of and infestations, as well as the interaction(s) between them. Therefore, this study aimed to identify the nematode population assemblages and endemic rhizosphere bacteria associated with soybean using Next Generation Sequencing (NGS). The abundance of bacterial genera that were then identified as being significant using linear discriminant analysis (LDA) Effect Size (LEfSe) was compared to the abundance of the most prevalent plant-parasitic nematode genera found across all sampled sites, . and . While several bacterial genera were identified as significant using LEfSe, only two with increased abundance were associated with decreased abundance of and . However, six bacterial genera were associated with decreased abundance. It is therefore possible that endemic bacterial strains can serve as an alternative method for reducing densities of plant-parasitic nematode genera and in this way reduce the damages caused to this economically important crop.
就产量而言，大豆是南非的主要作物之一。在过去几年里，植物寄生线虫感染对当地大豆造成的损害越来越大。大豆田中根结线虫和根腐线虫的存在会削弱该国的大豆产量，然而，对于与不同程度的根结线虫和根腐线虫侵染相关的大豆土壤微生物群落，以及它们之间的相互作用，人们了解甚少。因此，本研究旨在利用下一代测序（NGS）技术鉴定与大豆相关的线虫种群组合和地方性根际细菌。然后，使用线性判别分析（LDA）效应大小（LEfSe）确定为显著的细菌属的丰度，与在所有采样地点发现的最普遍的植物寄生线虫属的丰度进行比较，即根结线虫属和根腐线虫属。虽然使用LEfSe鉴定出几个显著的细菌属，但只有两个丰度增加的细菌属与根结线虫属和根腐线虫属丰度的降低有关。然而，有六个细菌属与根腐线虫属丰度的降低有关。因此，地方性细菌菌株有可能作为一种替代方法来降低植物寄生线虫属的密度，从而减少对这种经济上重要作物造成的损害。
