Sadeepa Dilini, Sirisena Kosala, Manage Pathmalal M
Centre for Water Quality and Algae Research, Department of Zoology, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka; Faculty of Graduate Studies, University of Sri Jayewardenepura, Nugegoda 10250, Sri Lanka.
Department of Environmental Technology, Faculty of Technology, University of Colombo, Sri Lanka.
Gene. 2022 Feb 20;812:146103. doi: 10.1016/j.gene.2021.146103. Epub 2021 Dec 9.
Characterization of hot spring microbiota is useful as an initial platform for exploring industrially important microbes. The present study focused on characterization of microbiota in four hot springs in Sri Lanka: Maha Oya; Wahava; Madunagala; and Kivlegama using high throughput 16S amplicon sequencing. Temperatures of the selected springs were ranged from 33.7 °C to 52.4 °C, whereas pH ranged from 7.2 to 8.2. Bacteria were found to be the dominant microbial group (>99%) compared to Archaea which represented less than 1% of microbiota. Four hot springs comprised of unique microbial community structures. Proteobacteria, Firmicutes, Bacteroidetes, Cloroflexi, Deinococcus and Actenobacteria were the major bacterial phyla. Moderately thermophilic genera such as Thermodesulfobacteria and Deinococcus-Thermus were detected as major genera that could be used in industrial applications operating at temperatures around 50 °C and alkaline reaction conditions.
温泉微生物群的表征作为探索具有工业重要性的微生物的初始平台很有用。本研究重点利用高通量16S扩增子测序对斯里兰卡四个温泉（马哈奥亚、瓦哈瓦、马杜纳加拉和基夫勒加马）的微生物群进行表征。所选温泉的温度范围为33.7℃至52.4℃，而pH值范围为7.2至8.2。与占微生物群不到1%的古菌相比，细菌是主要的微生物类群（>99%）。四个温泉具有独特的微生物群落结构。变形菌门、厚壁菌门、拟杆菌门、绿弯菌门、嗜热放线菌门和放线菌门是主要的细菌门类。检测到中度嗜热菌属，如嗜热脱硫杆菌属和嗜热栖热放线菌属，它们是可用于在50℃左右温度和碱性反应条件下运行的工业应用的主要菌属。
