New 8-Hydroxybriaranes from the Gorgonian Coral (Ellisellidae).

Three new 8-hydroxybriaranes-fragilides R-T (-) were obtained from a sea whip gorgonian coral . The structures of briaranes - were elucidated by using spectroscopic methods, including 1D (H and C NMR), 2D (COSY, HSQC, HMBC, and NOESY experiments) NMR studies, and (+)-HRESIMS. Fragilides S and T ( and ) are the only briaranes known to possess 8α-hydroxy and 17β-methyl groups, respectively. Briarane exerted an inhibition effect on iNOS release from RAW264.7; a macrophage cell line that originated from a mouse monocyte macrophage, stimulated with lipopolysaccharides.