Academy of Advanced Carbon Conversion Technology, Huaqiao University, Xiamen 361021, China.
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
Molecules. 2024 Sep 4;29(17):4201. doi: 10.3390/molecules29174201.
This study focused on developing an effective cell wall-breaking method for , followed by utilizing subcritical fluid extraction to isolate, extract, and concentrate astaxanthin from the complex fermentation products of . A comprehensive comparison of seven distinct methods for disrupting cell walls, including dimethyl sulfoxide treatment, lactic acid treatment, sodium hydroxide treatment, -glucanase enzymatic digestion, -mannanase enzymatic digestion, and a combined enzymatic treatment involving both -mannanase and -glucanase was conducted. The results identified the lactic acid method as the most effective in disrupting the cell walls of . The software, Design Expert, was used in the process of extracting astaxanthin from cell lysates using a subcritical extraction method. Through fitting analysis and response surface optimization analysis by Design Expert, the optimal extraction conditions were determined as follows: an extraction temperature of 41 °C, extraction frequency of two times, and extraction time of 46 min. These parameters facilitated the efficient extraction, concentration, and enrichment of astaxanthin from , resulting in an astaxanthin concentration of 540.00 mg/L. This result can establish the foundation for its high-value applications.
本研究专注于开发一种有效的破壁方法来处理 ，然后利用亚临界流体萃取从 的复杂发酵产物中分离、提取和浓缩虾青素。本文全面比较了七种不同的破壁方法，包括二甲基亚砜处理、乳酸处理、氢氧化钠处理、β-葡聚糖酶酶解、β-甘露聚糖酶酶解，以及同时涉及β-甘露聚糖酶和β-葡聚糖酶的联合酶处理。结果表明，乳酸法是处理 细胞壁最有效的方法。在使用亚临界萃取法从细胞裂解物中提取虾青素的过程中，使用了软件 Design Expert。通过 Design Expert 的拟合分析和响应面优化分析，确定了最佳提取条件如下：提取温度 41°C，提取频率两次，提取时间 46 分钟。这些参数促进了虾青素从 中的高效提取、浓缩和富集，虾青素浓度达到 540.00mg/L。这一结果为其高附加值应用奠定了基础。
