Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain; Universidad de Alcalá, Departamento de Química Analítica, Química Física e Ingeniería Química, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain.
Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain.
Food Chem. 2024 Oct 30;456:140034. doi: 10.1016/j.foodchem.2024.140034. Epub 2024 Jun 8.
Citrus reticulata L leaves are one of the main post-harvest byproduct, containing bioactive compounds, that are usually undervalued. This work describes the development of a biorefinery process based on the application of supercritical CO (SC-CO) followed by ultrasonic-assisted extraction (UAE) combined with Natural Deep Eutectic Solvents (NaDES) to extract bioactive terpenoids and phenolic compounds from these leaves. Extraction temperature and pressure of SC-CO were optimized, obtaining the highest bioactive terpenoids content using 200 bar at 60 °C. A Box-Behnken experimental design showed that 57% of water in NaDES composed of Choline Chloride and Glycerol (1:2) as extraction solvent at 25 °C for 50 min were the optimal UAE-NaDES extraction conditions to obtain the highest bioactive phenolic content from the residue of the optimal SC-CO extraction. The optimum extract presented the highest bioactivity and polyphenol content determined by LC-DAD-MS compared with extracts obtained using only water or NaDES as solvent.
柑橘叶是一种主要的采后副产物，含有生物活性化合物，但通常被低估了价值。本工作描述了一种基于超临界 CO（SC-CO）应用的生物炼制工艺的开发，随后是超声辅助提取（UAE）与天然深共晶溶剂（NaDES）相结合，从这些叶子中提取生物活性萜类化合物和酚类化合物。优化了 SC-CO 的提取温度和压力，在 60°C 和 200 巴的条件下获得了最高的生物活性萜类化合物含量。Box-Behnken 实验设计表明，NaDES 中 57%的水（由氯化胆碱和甘油组成，比例为 1:2）作为提取溶剂，在 25°C 下提取 50 分钟，是从最佳 SC-CO 提取残余物中获得最高生物活性酚含量的最佳 UAE-NaDES 提取条件。与仅用水或 NaDES 作为溶剂获得的提取物相比，最优提取物的 LC-DAD-MS 测定具有最高的生物活性和多酚含量。
