Biorational Pesticide Research Lab, Sichuan Agricultural University, Chengdu 611130, China.
Sichuan Provincial Plant Protection Station, Department of Agriculture, Chengdu 610041, China.
Int J Mol Sci. 2019 Sep 15;20(18):4573. doi: 10.3390/ijms20184573.
The white-back planthopper (WBPH), , is a major rice pest in China and in some other rice-growing countries of Asia. The extensive use of pesticides has resulted in severe resistance of to variety of chemical insecticides. Sulfoxaflor is a new diamide insecticide that acts on nicotinic acetylcholine receptors (nAChRs) in insects. The aim of this study was to explore the key genes related to the development of resistance to sulfoxaflor in and to verify their functions. Transcriptomes were compared between white-back planthoppers from a susceptible laboratory strain (Sus-Lab) and Sus-Lab screened with the sublethal LC dose of sulfoxaflor for six generations (SF-Sel). Two P450 genes ( and ) and three transcription factors (, and ) with upregulated expression verified by qRT-PCR were detected in the Sus-Lab and SF-Sel strains. The functions of and were analyzed by RNA interference, and the relative normalized expressions of and in the SF-Sel population were lower than under dsGFP treatment after dsRNA injection. Moreover, the mortality rates of SF-Sel population treated with the LC concentration of sulfoxaflor after the injecting of dsRNA targeting and were significantly higher than in the dsGFP group from 72 h to 96 h ( < 0.05), and mortality in the knockdown group was clearly higher than that of the knockdown group. The interaction between the tertiary structures of and and sulfoxaflor was also predicted, and showed a stronger metabolic ability to process sulfoxaflor. Therefore, overexpression of and may be one of the primary factors in the development of sulfoxaflor resistance in .
白背飞虱是中国和亚洲其他一些水稻种植国家的主要水稻害虫。由于大量使用农药， 对各种化学杀虫剂产生了严重的抗性。唑虫酰胺是一种新型双酰胺类杀虫剂，作用于昆虫烟碱型乙酰胆碱受体（nAChRs）。本研究旨在探讨 对唑虫酰胺产生抗性的关键基因，并验证其功能。通过比较敏感的实验室品系（Sus-Lab）和经过亚致死剂量唑虫酰胺筛选 6 代的品系（SF-Sel）的转录组，发现了两个 P450 基因（ 和 ）和三个转录因子（ 、 和 ）在 Sus-Lab 和 SF-Sel 菌株中表达上调，通过 qRT-PCR 验证。通过 RNA 干扰分析了 和 的功能，在 SF-Sel 种群中，dsRNA 注射后 和 的相对正常表达水平低于 dsGFP 处理。此外，在 LC 浓度的唑虫酰胺处理后，靶向 和 的 dsRNA 注射的 SF-Sel 种群的死亡率明显高于 dsGFP 组，从 72 h 到 96 h（ < 0.05），且 敲低组的死亡率明显高于 敲低组。还预测了 和 与唑虫酰胺的三级结构之间的相互作用， 显示出更强的代谢能力来处理唑虫酰胺。因此， 和 的过表达可能是 对唑虫酰胺产生抗性的主要因素之一。
