Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
UMR PHAN, INRA & Université de Nantes, IMAD, CRNH-Ouest, Nantes, France.
Mol Biol Rep. 2019 Dec;46(6):5685-5693. doi: 10.1007/s11033-019-05000-5. Epub 2019 Sep 21.
Metabolic syndrome (MetS) results from the interaction between environmental and genetic factors. Several previous studies considered the role of selenium in developing MetS. Two selenoproteins, selenoprotein S (SelS), and the Selenoprotein P (SePP) play an important role in antioxidative defense and therefore susceptibility to MetS. The involvement of SNPs in SEPP1 and SEPS1 have not been studied in MetS subjects. This study aims to investigate the association between the risk of MetS and four polymorphisms SEPS1 (rs28665122, rs4965373), SEPP1 (rs7579, rs3877899) in an Iranian population. The sample of this case-control study consisted of 132 Iranian patients with cardiovascular disease (71 MetS and 65 non-MetS subjects) from December 2015 to March 2016. Demographic data, medical history, and para-clinical were measured, and Taqman probes were used for allelic discrimination. The level of the SelS and the SePP were measured by the ELIZA method. No significant differences were found in the genotype frequencies of SEPS1 (rs4965373, rs28665122), SEPP1 (rs7579, rs3877899) in patients with MetS and the non-MetS group. The mean of SelS in MetS subjects with SEPS1 (rs4965373) GG genotype is significantly lower than the non-MetS group (4496.99 ± 3688.5 vs. 6148.6 ± 1127.0, P = 0.009). The mean of SePP in MetS subjects with SEPP1 (rs3877899) GG genotype is significantly lower than the non-MetS group (40.73 ± 8.44 vs.83.91 ± 21.33, P = 0.002). The mean of SePP in MetS subjects with SEPP1 (rs7579) GG genotype is lower than the non-MetS group (55.52 ± 16.7 vs. 109.48 ± 29.78, P = 0.01). In summary, the results of this study does not indicate significant differences in the SEPP1 (rs7579, rs3877899) and SEPS1 (rs4965373, rs28665122) genotypes between MetS and non-MetS subjects. However, the results show that the mean of expression of SelS and SePP decreased in the subjects with SEPP1 (rs7579) GG and SEPP1 (rs3877899) GG.
代谢综合征（MetS）是由环境和遗传因素相互作用引起的。先前有几项研究考虑了硒在代谢综合征发展中的作用。两种硒蛋白，硒蛋白 S（SelS）和硒蛋白 P（SePP）在抗氧化防御中发挥重要作用，因此易患代谢综合征。SEPP1 和 SEPS1 中 SNP 的参与在代谢综合征患者中尚未得到研究。本研究旨在探讨伊朗人群中 SEPS1（rs28665122、rs4965373）和 SEPP1（rs7579、rs3877899）四个多态性与代谢综合征风险之间的关联。这项病例对照研究的样本包括 2015 年 12 月至 2016 年 3 月期间 132 名患有心血管疾病的伊朗患者（71 名代谢综合征患者和 65 名非代谢综合征患者）。测量了人口统计学数据、病史和临床前数据，并使用 Taqman 探针进行等位基因鉴别。使用 ELISA 法测量 SelS 和 SePP 的水平。代谢综合征患者与非代谢综合征组在 SEPS1（rs4965373、rs28665122）和 SEPP1（rs7579、rs3877899）基因型频率方面无显著差异。代谢综合征患者 SEPS1（rs4965373）GG 基因型 SelS 的平均值明显低于非代谢综合征组（4496.99±3688.5 与 6148.6±1127.0，P=0.009）。代谢综合征患者 SEPP1（rs3877899）GG 基因型 SePP 的平均值明显低于非代谢综合征组（40.73±8.44 与 83.91±21.33，P=0.002）。代谢综合征患者 SEPP1（rs7579）GG 基因型的 SePP 平均值低于非代谢综合征组（55.52±16.7 与 109.48±29.78，P=0.01）。综上所述，本研究结果表明，代谢综合征与非代谢综合征患者之间 SEPP1（rs7579、rs3877899）和 SEPS1（rs4965373、rs28665122）基因型无显著差异。然而，结果表明，SEPP1（rs7579）GG 和 SEPP1（rs3877899）GG 患者 SelS 和 SePP 的表达平均值降低。
