Department of General Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Department of General Surgery, The second people's Hospital of Jiangyin, Jiangyin, P.R. China.
Int J Biol Markers. 2019 Dec;34(4):348-355. doi: 10.1177/1724600819876951. Epub 2019 Sep 23.
The oncogene IQ motif-containing GTPase activating protein 3 (IQGAP3) is ubiquitously overexpressed in several human cancers. This study was designed to explore the expression and role of IQGAP3 in colorectal cancer.
We first assessed the IQGAP3 expression level in colorectal cancer. The correlation of IQGAP3 expression with the clinicopathological characteristics and prognosis was then assessed. At last, we investigated the function of IQGAP3 in colorectal cancer by knocking down its expression in colorectal cancer cell lines.
Consistent with the conclusions drawn from The Cancer Genome Atlas database, IQGAP3 was upregulated in colorectal cancer at the tissue level and cellular level. Based on immunohistochemistry results of the tissue microarrays, we demonstrated that higher expression of IQGAP3 was associated with higher tumor node metastasis stage ( = 0.005), higher incidence of lymph node metastasis ( = 0.004), and shorter overall survival ( = 0.022). Knockdown of IQGAP3 in colorectal cancer cell lines remarkably decreased their proliferation and migration abilities.
Our data provide evidence that IQGAP3 significantly promote malignant progression of colorectal cancer and could serve as a potential therapeutic target.
原癌基因 IQ 基序富含 GTP 酶激活蛋白 3（IQGAP3）在几种人类癌症中广泛过表达。本研究旨在探讨 IQGAP3 在结直肠癌中的表达和作用。
我们首先评估了结直肠癌中 IQGAP3 的表达水平。然后评估了 IQGAP3 表达与临床病理特征和预后的相关性。最后，我们通过敲低结直肠癌细胞系中的 IQGAP3 表达来研究 IQGAP3 在结直肠癌中的功能。
与癌症基因组图谱数据库的结论一致，IQGAP3 在结直肠癌组织和细胞水平上均上调。基于组织微阵列的免疫组化结果，我们证明 IQGAP3 的高表达与更高的肿瘤淋巴结转移分期（=0.005）、更高的淋巴结转移发生率（=0.004）和更短的总生存期（=0.022）相关。敲低结直肠癌细胞系中的 IQGAP3 显著降低了它们的增殖和迁移能力。
我们的数据提供了证据表明 IQGAP3 显著促进了结直肠癌的恶性进展，可作为潜在的治疗靶点。
