One pot facile green synthesis of crystalline bio-ZrO nanoparticles using Acinetobacter sp. KCSI1 under room temperature.

In this study, crystalline ZrO nanoparticles were synthesized in one pot at room temperature using an extremophilic Acinetobacter sp. KCSI1 and characterized using various techniques to study its structural, optical and crystalline properties. The average size of the ZrO nanoparticles was found to be 44 ± 7 nm. The XRD and Raman spectra showed the crystalline structure of ZrO HRTEM and SAED images showed well aligned ordered crystal lattice nanoparticles. The zeta potential of Bio-NP of ZrO has been found to be 36.5 ± 5.46 mV. The mechanical behaviour such as hardness and Young's modulus of Bio-ZrO NPs were determined using atomic force microscopy (AFM) and found to be 9.206 ± 2.22 and 0.285 ± 0.13 GPa, respectively. No significant cytotoxicity for ZrO nanoparticles was observed when tested with mouse fibroblast cells (L929), suggesting that the synthesized ZrO nanoparticle is biocompatible and safe for environmental applications.